Name: Biotinylated Human CD133 Nanodisc (CD1-HM13NB)
Brand: KactusBio
SKU: CD1-HM13NB-100UG
Price: 2166.0 USD
Availability: InStock

Biotinylated Human CD133 Nanodisc (CD1-HM13NB)

Biotinylated Biotinylated Human CD133 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-CD133 Antibody, hFc Tag with an affinity constant of 26.57 pM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Human CD133 Nanodisc, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD133 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.

Express System: HEK293
Product Tag: C-His
Activity: Immobilized Biotinylated Human CD133 Nanodisc, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD133 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA. The affinity constant of 26.57 pM as determined in SPR assay (Biacore T200).
Recomended Products: Biotinylated Nanodisc Control (CON-HM1NB)
Amino Acid Range: Met1-His865

Catalog #CD1-HM13NB

Size

100UG

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Biotinylated Human CD133 Nanodisc (CD1-HM13NB) Summary

Product Description	Recombinant Biotinylated Human CD133 Nanodisc is expressed from HEK293 with His tag at the C-terminus.It contains Met1-His865.
Product Background	Prominin-1 (PROM1), also known as CD133, is expressed in hepatic progenitor cells (HPCs) and cholangiocytes of the fibrotic liver. It is a cell surface biomarker that allows the identification of stem and cancer stem cells from different organs. It is also expressed in several differentiated epithelial and non-epithelial cells.
Product Category	Recombinant Protein / Antibody discovery / Multiple transmembrane proteins / Nanodisc

Biotinylated Human CD133 Nanodisc (CD1-HM13NB) Specifications/Details

Protein	CD133
Synonyms	PROM1; PROML1; MSTP061; Prominin-1; AC133
Accession	O43490-1
Species	Human
Biotinylated	yes
Amino Acid Range	Met1-His865
Molecular Weight	The protein has a predicted MW of 110.1 kDa.
Activity	Immobilized Biotinylated Human CD133 Nanodisc, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD133 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA. The affinity constant of 26.57 pM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Expression System	HEK293
Endotoxin	Less than 1EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Stability And Storage	Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Biotinylated Biotinylated Human CD133 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-CD133 Antibody, hFc Tag with an affinity constant of 26.57 pM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human CD133 Nanodisc, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD133 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.