Product Description |
Recombinant Biotinylated Human CD155/PVR Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Trp21-Asn343. |
Product Background |
CD155 is a cell surface adhesion molecule functioning in tumor cell migration, invasion, and metastasis, and not surprisingly, is also designated as a common tumor-associated antigen. CD155 is also recognized by NK cells to induce their cytotoxicity. CD155 is also commonly referred to as the "poliovirus receptor," or PVR. |
Product Category |
Recombinant Protein |
Protein |
CD155/PVR |
Synonyms |
CD155; HVED; NECL5; Necl-5; nectin-like 5; PVR; PVS; Tage4; PVSFLJ25946; FLJ25946 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Trp21-Asn343 |
Molecular Weight |
The protein has a predicted MW of 38 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human CD155, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Biotinylated Human CD155, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). |
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Immobilized Biotinylated Human CD155, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human DNAM-1, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA. |
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Biotinylated Human CD155 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
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The purity of Biotinylated Human CD155 is greater than 95% as determined by SEC-HPLC. |
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