Biotinylated Human CD155/PVR Protein (CD1-HM455B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human CD155, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Trp21-Asn343

Catalog: CD1-HM455B

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Product Description Recombinant Biotinylated Human CD155/PVR Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Trp21-Asn343.
Product Category Recombinant Protein
Target CD155/PVR
Synonyms CD155; HVED; NECL5; Necl-5; nectin-like 5; PVR; PVS; Tage4; PVSFLJ25946; FLJ25946
Gene/Protein ID P15151-1
Species Human
Exact Sequence Trp21-Asn343
Molecular Weight The protein has a predicted MW of 38 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Human CD155, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human CD155, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA.
The purity of Biotinylated Human CD155 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human CD155 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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