- Express System: HEK293
- Product Tag: C-His-Avi
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Anti-CD161 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD161, His Tag with the EC50 of 32.5ng/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Gln67-Ser225
Product Description |
Recombinant Biotinylated Human CD161 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln67-Ser225. |
Product Background |
CD161 (NKRP1) is a lectin-like receptor present on NK cells and rare T-cell subsets. We have observed CD161 expression in some cases of T-cell prolymphocytic leukemia (T-PLL) and found it to be useful in follow-up and detection of disease after treatment. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Protein |
CD161 |
Synonyms |
KLRB1; CLEC5B; NKRP1A; NKR-P1A; HNKR-P1a; CD161; Ly59; NKR; NKRP1; NKR-P1; NKRP1ANKR |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gln67-Ser225 |
Molecular Weight |
The protein has a predicted MW of 21.4 kDa. Due to glycosylation, the protein migrates to 41-45 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Anti-CD161 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD161, His Tag with the EC50 of 32.5ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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