Name: Biotinylated Human CD20/MS4A1 Nanodisc (CD2-HM12NB)
Brand: KactusBio
SKU: CD2-HM12NB-100UG
Price: 2165.9 USD
Availability: InStock

Biotinylated Human CD20/MS4A1 Nanodisc (CD2-HM12NB)

Biotinylated Human CD20 Nanodisc captured on CM5 Chip via Streptavidin can bind Rituximab, hFc Tag with an affinity constant of 10.21 nM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Human CD20 Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Ofatumumab, hFc Tag with the EC50 of 30.8ng/ml determined by ELISA.

Immobilized Biotinylated Human CD20 Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Rituximab, hFc Tag with the EC50 of 0.16ug/ml determined by ELISA.

Immobilized Biotinylated Human CD20 Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Obinutuzumab, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA.

Loaded Biotinylated Human CD20 Nanodisc, His Tag on Streptavidin-Biosensor can bind Rituximab, hFc Tag with an affinity constant of 6.94 nM as determined in BLI assay (Gator® Prime).

Express System: HEK293
Product Tag: C-His
Activity: Immobilized Biotinylated Human CD20 Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Ofatumumab, hFc Tag with the EC50 of 30.8ng/ml determined by ELISA. The affinity constant of 10.21 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Met1-Pro297

Catalog #CD2-HM12NB

Size

100UG

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Biotinylated Human CD20/MS4A1 Nanodisc (CD2-HM12NB) Summary

Product Description	Recombinant Biotinylated Human CD20/MS4A1 Nanodisc is expressed from HEK293 with His tag at the C-terminus.It contains Met1-Pro297.
Product Background	B-lymphocyte antigen CD20 or CD20 is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R , CD117) and progressively increasing in concentration until maturity.CD20 is the target of the monoclonal antibodies rituximab, ocrelizumab, obinutuzumab, ofatumumab, ibritumomab tiuxetan, tositumomab, and ublituximab, which are all active agents in the treatment of all B cell lymphomas, leukemias, and B cell-mediated autoimmune diseases.
Product Category	Recombinant Protein / Antibody discovery / Multiple transmembrane proteins / Nanodisc

Biotinylated Human CD20/MS4A1 Nanodisc (CD2-HM12NB) Specifications/Details

Protein	CD20/MS4A1
Synonyms	MS4A1; CD20; MS4A-1; B1; Bp35; CD20 receptor; CD20S7; CVID5; LEU-16; Ly-44; MS4A2; S7
Accession	P11836-1
Species	Human
Biotinylated	yes
Amino Acid Range	Met1-Pro297
Molecular Weight	The protein has a predicted MW of 46.0 kDa.
Activity	Immobilized Biotinylated Human CD20 Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Ofatumumab, hFc Tag with the EC50 of 30.8ng/ml determined by ELISA. The affinity constant of 10.21 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS, 200mM L-Arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Stability And Storage	Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Biotinylated Human CD20 Nanodisc captured on CM5 Chip via Streptavidin can bind Rituximab, hFc Tag with an affinity constant of 10.21 nM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human CD20 Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Ofatumumab, hFc Tag with the EC50 of 30.8ng/ml determined by ELISA.
	Immobilized Biotinylated Human CD20 Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Rituximab, hFc Tag with the EC50 of 0.16ug/ml determined by ELISA.
	Immobilized Biotinylated Human CD20 Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Obinutuzumab, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA.
	Loaded Biotinylated Human CD20 Nanodisc, His Tag on Streptavidin-Biosensor can bind Rituximab, hFc Tag with an affinity constant of 6.94 nM as determined in BLI assay (Gator® Prime).