• Express System: HEK293
  • Purity: > 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human CD20 VLP at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Rituximab, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.40 nM as determined in SPR assay (Biacore T200).
  • Negative Control: Biotinylated VLP Control (GPR-HM05CB)
  • Amino Acid Range: Met1-Pro297
Catalog #CD2-HM123B
Price
Regular price $1,76500
Regular price Sale price $1,76500
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human CD20/MS4A1 VLP (CD2-HM123B) Summary

Product Description

 Recombinant Biotinylated Human CD20/MS4A1 VLP is expressed from HEK293.It contains Met1-Pro297 (It may have cross reaction with anti-His antibody).

Product Background

 B-lymphocyte antigen CD20 or CD20 is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R , CD117) and progressively increasing in concentration until maturity.CD20 is the target of the monoclonal antibodies rituximab, ocrelizumab, obinutuzumab, ofatumumab, ibritumomab tiuxetan, tositumomab, and ublituximab, which are all active agents in the treatment of all B cell lymphomas, leukemias, and B cell-mediated autoimmune diseases.

Biotinylated Human CD20/MS4A1 VLP (CD2-HM123B) Specifications/Details

Protein
 CD20/MS4A1
Synonyms
 MS4A1; CD20; MS4A-1; B1; Bp35; CD20 receptor; CD20S7; CVID5; LEU-16; Ly-44; MS4A2; S7;LEU16; Ly44
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Met1-Pro297
Molecular Weight
 The target protein has a predicted MW of 34.3 kDa.
Activity
 Immobilized Biotinylated Human CD20 VLP at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Rituximab, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.40 nM as determined in SPR assay (Biacore T200).
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22um filtered solution in PBS, 300mM L-arginine (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Biotinylated Human CD20 VLP captured on CM5 Chip via streptavidin can bind Rituximab, hFc Tag with an affinity constant of 0.40 nM as determined in SPR assay (Biacore T200). Biotinylated Human CD20 VLP captured on CM5 Chip via streptavidin can bind Rituximab, hFc Tag with an affinity constant of 0.40 nM as determined in SPR assay (Biacore T200).
Biotinylated Human CD20 VLP captured on CM5 Chip via streptavidin can bind Ofatumumab, hFc Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). Biotinylated Human CD20 VLP captured on CM5 Chip via streptavidin can bind Ofatumumab, hFc Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human CD20 VLP at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Rituximab, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human CD20 VLP at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Rituximab, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human CD20 VLP at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Ofatumumab, hFc Tag with the EC50 of 13.9ng/ml determined by ELISA. Immobilized Biotinylated Human CD20 VLP at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Ofatumumab, hFc Tag with the EC50 of 13.9ng/ml determined by ELISA.
Immobilized Biotinylated Human CD20 VLP at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Obinutuzumab, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA. Immobilized Biotinylated Human CD20 VLP at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Obinutuzumab, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA.
1E6 of Anti-CD20 CAR-HEK293T cells were stained with 100 ul of 5 ug/ml VLP (Cat. CD2-HM123B). Irrelevant VLP protein were used as negative control. PE-SA signal was used to evaluate the binding activity. 1E6 of Anti-CD20 CAR-HEK293T cells were stained with 100 ul of 5 ug/ml VLP (Cat. CD2-HM123B). Irrelevant VLP protein were used as negative control. PE-SA signal was used to evaluate the binding activity.
The purity of Biotinylated Human CD20 VLP is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human CD20 VLP is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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