Name: Biotinylated Human CD228/MFI2 Protein (MFI-HM428B)
Brand: KactusBio
SKU: MFI-HM428B-100UG
Price: 578.0 USD
Availability: InStock

Biotinylated Human CD228/MFI2 Protein (MFI-HM428B)

Immobilized Biotinylated Human CD228, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD228 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA.

Biotinylated Human CD228 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human CD228 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human CD228, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD228 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gly20-Cys709

Catalog #MFI-HM428B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human CD228/MFI2 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly20-Cys709.
Product Background	Melanotransferrin is typically overexpressed in melanoma cells compared to other cell types - including cancer cells - and is efficiently sorted and secreted with nanovesicles, or so-called exosomes, due to its membrane-anchoring by a glycosylphosphatidylinositol. Melanotransferrin is exposed on the surface of exosomes and is accessible for antibody recognition.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	CD228/MFI2
Synonyms	Melanotransferrin; CD228; MFI2; MELTF; MAP97; MFI2
Accession	P08582-1
Species	Human
Biotinylated	yes
Amino Acid Range	Gly20-Cys709
Molecular Weight	The protein has a predicted MW of 78.42 kDa. Due to glycosylation, the protein migrates to 80-90 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human CD228, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD228 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human CD228, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD228 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA.
	Biotinylated Human CD228 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human CD228 is greater than 95% as determined by SEC-HPLC.