Name: Biotinylated Human CD228/MFI2 Protein (MFI-HM428B)
Brand: KactusBio
SKU: MFI-HM428B-100UG
Price: 578.0 USD
Availability: InStock

Biotinylated Human CD228/MFI2 Protein (MFI-HM428B)

Immobilized Biotinylated Human CD228, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD228 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA.

Biotinylated Human CD228 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human CD228 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human CD228, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD228 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA.
Amino Acid Range: Gly20-Cys709

Catalog #MFI-HM428B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human CD228/MFI2 Protein (MFI-HM428B) Summary

Product Description	Recombinant Biotinylated Human CD228/MFI2 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly20-Cys709.
Product Background	Melanotransferrin is typically overexpressed in melanoma cells compared to other cell types - including cancer cells - and is efficiently sorted and secreted with nanovesicles, or so-called exosomes, due to its membrane-anchoring by a glycosylphosphatidylinositol. Melanotransferrin is exposed on the surface of exosomes and is accessible for antibody recognition.
Product Category	Recombinant Protein / Antibody discovery / ADC targets

Biotinylated Human CD228/MFI2 Protein (MFI-HM428B) Specifications/Details

Protein	CD228/MFI2
Synonyms	Melanotransferrin; CD228; MFI2; MELTF; MAP97; MFI2
Accession	P08582-1
Species	Human
Biotinylated	yes
Amino Acid Range	Gly20-Cys709
Molecular Weight	The protein has a predicted MW of 78.42 kDa. Due to glycosylation, the protein migrates to 80-90 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human CD228, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD228 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human CD228, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD228 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA.
	Biotinylated Human CD228 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human CD228 is greater than 95% as determined by SEC-HPLC.