Name: Biotinylated Human CD30/TNFRSF8 Protein, Ultra Low Endotoxin (CD3-HM430B-UL)
Brand: KactusBio
SKU: CD3-HM430B-UL-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human CD30/TNFRSF8 Protein, Ultra Low Endotoxin (CD3-HM430B-UL)

Immobilized Human CD30 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD30, His Tag with the EC50 of 17.5ng/ml determined by ELISA (QC Test).

Immobilized Anti-CD30 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD30, His Tag with the EC50 of 11.5ng/ml determined by ELISA.

Biotinylated CD30 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated CD30 is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human CD30 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD30, His Tag with the EC50 of 17.5ng/ml determined by ELISA (QC Test).
Amino Acid Range: Phe19-Lys379

Catalog #CD3-HM430B-UL

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human CD30/TNFRSF8 Protein, Ultra Low Endotoxin (CD3-HM430B-UL) Summary

Product Description	Recombinant Biotinylated Human CD30/TNFRSF8 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe19-Lys379.
Product Background	The transmembrane receptor CD30 (TNFRSF8) and its ligand CD30L (CD153, TNFSF8) are members of the tumor necrosis factor (TNF) superfamily and display restricted expression in subpopulations of activated T-and B-cells in nonpathologic conditions. CD30 expression is upregulated in various hematological malignancies, including Reed-Sternberg cells in Hodgkin's disease (HD), anaplastic large cell lymphoma (ALCL) and subsets of Non-Hodgkin's lymphomas (NHLs).
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets \| ADC targets

Biotinylated Human CD30/TNFRSF8 Protein, Ultra Low Endotoxin (CD3-HM430B-UL) Specifications/Details

Protein	CD30/TNFRSF8
Synonyms	CD30; CD30KI-1; CD30L receptor; TNFRSF8; D1S166EKi-1; CD30KI-1
Accession	P28908-1
Species	Human
Biotinylated	yes
Amino Acid Range	Phe19-Lys379
Molecular Weight	The protein has a predicted MW of 41.3 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD30 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD30, His Tag with the EC50 of 17.5ng/ml determined by ELISA (QC Test).
Product Tag	C-His-Avi
Fluorophore	Biotin-labeled
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human CD30 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD30, His Tag with the EC50 of 17.5ng/ml determined by ELISA (QC Test).
	Immobilized Anti-CD30 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD30, His Tag with the EC50 of 11.5ng/ml determined by ELISA.
	Biotinylated CD30 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated CD30 is greater than 90% as determined by SEC-HPLC.

Other Available Proteins:

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Biotinylated Human CD30/TNFRSF8 Protein, Ultra Low Endotoxin (CD3-HM430B-UL)

Biotinylated Human CD30/TNFRSF8 Protein, Ultra Low Endotoxin (CD3-HM430B-UL)

Biotinylated Human CD30/TNFRSF8 Protein, Ultra Low Endotoxin (CD3-HM430B-UL) Summary

Product Description

Product Background

Product Category

Biotinylated Human CD30/TNFRSF8 Protein, Ultra Low Endotoxin (CD3-HM430B-UL) Specifications/Details

Fluorophore

Shipping, Reconstitution, & Storage

Validation Images

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