Product Description |
Recombinant Biotinylated Human CD300A Protein is expressed from HEK293 with Avi tag at the C-terminus.It contains Leu18-Pro180. |
Product Background |
CD300a is an inhibitory receptor for mast cells and eosinophils in allergic inflammation (AI); however, the spatiotemporal expression of CD300a and its potential roles in the resolution of AI are still to be determined.CD300a expression on peritoneal cells is regulated from inflammation to resolution. CD300a activation on mouse bone marrow-derived mast cells regulated ALX/FPR2 expression levels following IgE-mediated activation. |
Product Category |
Recombinant Protein |
Target |
CD300A |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Leu18-Pro180 |
Molecular Weight |
The protein has a predicted MW of 19.46 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human CD300A, Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD300A Antibody, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Biotinylated Human CD300A, Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD300A Antibody, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA (QC Test). |
|
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Biotinylated Human CD300A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Biotinylated Human CD300A is greater than 95% as determined by SEC-HPLC. |
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