Name: Biotinylated Human CD3E/CD3 epsilon Protein (CDE-HM401B)
Brand: KactusBio
SKU: CDE-HM401B-100UG
Price: 578.0 USD
Availability: InStock

Biotinylated Human CD3E/CD3 epsilon Protein (CDE-HM401B)

Immobilized Biotinylated Human CD3E, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA.

Biotinylated Human CD3E on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Biotinylated Human CD3E, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Asp23-Asp126

Catalog #CDE-HM401B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human CD3E/CD3 epsilon Protein (CDE-HM401B) Summary

Product Description	Recombinant Biotinylated Human CD3E/CD3 epsilon Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Asp23-Asp126.
Product Background	CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human CD3E/CD3 epsilon Protein (CDE-HM401B) Specifications/Details

Protein	CD3E/CD3 epsilon
Synonyms	CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18
Accession	P07766
Species	Human
Biotinylated	yes
Amino Acid Range	Asp23-Asp126
Molecular Weight	The protein has a predicted MW of 14.66 kDa. Due to glycosylation, the protein migrates to 16-20 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human CD3E, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human CD3E, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA.
	Biotinylated Human CD3E on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.