Biotinylated Human CD3E/CD3 epsilon Protein (CDE-HM401B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Biotinylated Human CD3E, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Asp23-Asp126
Catalog #CDE-HM401B
Regular price $578.00
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Biotinylated Human CD3E/CD3 epsilon Protein (CDE-HM401B) Summary

Product Description

Recombinant Biotinylated Human CD3E/CD3 epsilon Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Asp23-Asp126.

Product Background

CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Biotinylated Human CD3E/CD3 epsilon Protein (CDE-HM401B) Specifications/Details

Protein
CD3E/CD3 epsilon
Synonyms
CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Asp23-Asp126
Molecular Weight
The protein has a predicted MW of 14.66 kDa. Due to glycosylation, the protein migrates to 16-20 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human CD3E, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human CD3E, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA. Immobilized Biotinylated Human CD3E, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA.
Biotinylated Human CD3E on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human CD3E on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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