Biotinylated Human CD3E/CD3 epsilon Protein, Ultra Low Endotoxin (CDE-HM401B-UL)

SKU: CDE-HM401B-UL-100UG

Biotinylated Human CD3E/CD3 epsilon Protein, Ultra Low Endotoxin (CDE-HM401B-UL)

SKU: CDE-HM401B-UL-100UG
Biotinylated Human CD3E/CD3 epsilon Protein, Ultra Low Endotoxin (CDE-HM401B-UL)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Biotinylated Human CD3E, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA.
  • Amino Acid Range: Asp23-Asp126
Catalog #CDE-HM401B-UL
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Size
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Biotinylated Human CD3E/CD3 epsilon Protein, Ultra Low Endotoxin (CDE-HM401B-UL) Summary

Product Description

 Recombinant Biotinylated Human CD3E/CD3 epsilon Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Asp23-Asp126.

Product Background

 CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Biotinylated Human CD3E/CD3 epsilon Protein, Ultra Low Endotoxin (CDE-HM401B-UL) Specifications/Details

Protein
 CD3E/CD3 epsilon
Synonyms
 CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Asp23-Asp126
Molecular Weight
 The protein has a predicted MW of 14.66 kDa. Due to glycosylation, the protein migrates to 16-20 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human CD3E, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA.
Product Tag
 C-His-Avi

Fluorophore

 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human CD3E, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA. Immobilized Biotinylated Human CD3E, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA.
Biotinylated Human CD3E on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human CD3E on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...
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