Name: Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-HM405B)
Brand: KactusBio
SKU: CD3-HM405B-100UG
Price: 660.0 USD
Availability: InStock

Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-HM405B)

Immobilized Biotinylated Human CD3E&CD3D, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 42.0ng/ml determined by ELISA.

Immobilized Biotinylated Human CD3E&CD3D, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 15.1ng/ml determined by ELISA.

Biotinylated Human CD3E&CD3D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human CD3E&CD3D is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human CD3E&CD3D, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 42.0ng/ml determined by ELISA.
Amino Acid Range: Asp23-Asp126 (CD3E) & Phe22-Ala105 (CD3D)

Catalog #CD3-HM405B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-HM405B) Summary

Product Description	Recombinant Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus of CD3D.It contains Asp23-Asp126 (CD3E) and Phe22-Ala105 (CD3D).
Product Background	T-cell surface glycoprotein CD3 epsilon&CD3 delta chain, also known as CD3E&CD3D, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-HM405B) Specifications/Details

Protein	CD3E&CD3D/CD3 epsilon&CD3 delta
Synonyms	CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon
Accession	P07766(CD3E)&P04234(CD3D)
Species	Human
Biotinylated	yes
Amino Acid Range	Asp23-Asp126 (CD3E) & Phe22-Ala105 (CD3D)
Molecular Weight	The protein has a predicted MW of 17.9 kDa (CD3E) and 18.52 kDa (CD3D). Due to glycosylation, the protein migrates to 23-27 kDa and 32-40 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human CD3E&CD3D, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 42.0ng/ml determined by ELISA.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human CD3E&CD3D, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 42.0ng/ml determined by ELISA.
	Immobilized Biotinylated Human CD3E&CD3D, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 15.1ng/ml determined by ELISA.
	Biotinylated Human CD3E&CD3D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human CD3E&CD3D is greater than 95% as determined by SEC-HPLC.