Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) (CD3-HM105B)
Catalog: CD3-HM105B
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| Product Description | Recombinant Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) is expressed from Expi293 with His tag at the C-terminal.It contains Asp23-Glu120(CD3E) & Phe22-Ala105(CD3D). |
| Product Category | Recombinant Protein |
| Target | CD3E&CD3D/CD3 epsilon&CD3 delta |
| Gene/Protein ID | P07766(CD3E)&P04234(CD3D) |
| Species | Human |
| Exact Sequence | Asp23-Glu120(CD3E) & Phe22-Ala105(CD3D) |
| Molecular Weight | The protein has a predicted MW of 17.9 kDa (CD3E) and 16.7 kDa (CD3D). Due to glycosylation, the protein migrates to 25-40 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Anti-CD3 Antibody, mFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD3E&CD3D, His Tag with the EC50 of 0.14μg/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Anti-CD3 Antibody, mFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD3E&CD3D, His Tag with the EC50 of 0.14μg/ml determined by ELISA. |
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The purity of Biotinylated Human CD3E&CD3D is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human CD3E&CD3D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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