Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) (CD3-HM105B)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-CD3 Antibody, mFc Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human CD3E&CD3D, His Tag with the EC50 of 0.72 ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Asp23-Glu120 (CD3E) & Phe22-Ala105 (CD3D)

Catalog: CD3-HM105B

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Product Description Recombinant Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.; It contains Asp23-Glu120 (CD3E)&Phe22-Ala105 (CD3D).

Product Background

T-cell surface glycoprotein CD3 epsilon&CD3 delta chain, also known as CD3E&CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.

Product Category

Recombinant Protein

Target

CD3E&CD3D/CD3 epsilon&CD3 delta
Accession

Species

Human

Amino Acid Range

Asp23-Glu120 (CD3E) & Phe22-Ala105 (CD3D)
Molecular Weight The protein has a predicted MW of 17.9 kDa (CD3E) and 16.7 kDa (CD3D). Due to glycosylation, the protein migrates to 25-40 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Anti-CD3 Antibody, mFc Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human CD3E&CD3D, His Tag with the EC50 of 0.72 ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1 EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-CD3 Antibody, mFc Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human CD3E&CD3D, His Tag with the EC50 of 0.72 ug/ml determined by ELISA.
The purity of Biotinylated Human CD3E&CD3D is greater than 95% as determined by SEC-HPLC.
Biotinylated Human CD3E&CD3D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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