Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) (CD3-HM105B)
Catalog: CD3-HM105B
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Product Description | Recombinant Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Asp23-Glu120(CD3E) & Phe22-Ala105(CD3D). |
Product Category | Recombinant Protein |
Target | CD3E&CD3D/CD3 epsilon&CD3 delta |
Gene/Protein ID | P07766(CD3E)&P04234(CD3D) |
Species | Human |
Exact Sequence | Asp23-Glu120(CD3E) & Phe22-Ala105(CD3D) |
Molecular Weight | The protein has a predicted MW of 17.9 kDa (CD3E) and 16.7 kDa (CD3D). Due to glycosylation, the protein migrates to 25-40 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Anti-CD3 Antibody, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD3E&CD3D, His Tag with the EC50 of 0.72ug/ml determined by ELISA. See testing image for detail. |
Product Tag | C-His |
Express System | HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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