Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) (CD3-HM105B)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-CD3 Antibody, mFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD3E&CD3D, His Tag with the EC50 of 0.14μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Asp23-Glu120(CD3E) & Phe22-Ala105(CD3D)

Catalog: CD3-HM105B

$750.00
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Product Description Recombinant Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) is expressed from Expi293 with His tag at the C-terminal.It contains Asp23-Glu120(CD3E) & Phe22-Ala105(CD3D).
Product Category Recombinant Protein
Target CD3E&CD3D/CD3 epsilon&CD3 delta
Gene/Protein ID P07766(CD3E)&P04234(CD3D)
Species Human
Exact Sequence Asp23-Glu120(CD3E) & Phe22-Ala105(CD3D)
Molecular Weight The protein has a predicted MW of 17.9 kDa (CD3E) and 16.7 kDa (CD3D). Due to glycosylation, the protein migrates to 25-40 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-CD3 Antibody, mFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD3E&CD3D, His Tag with the EC50 of 0.14μg/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-CD3 Antibody, mFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD3E&CD3D, His Tag with the EC50 of 0.14μg/ml determined by ELISA.
The purity of Biotinylated Human CD3E&CD3D is greater than 95% as determined by SEC-HPLC.
Biotinylated Human CD3E&CD3D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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