Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) (CD3-HM105B)
Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) (CD3-HM105B)
Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) (CD3-HM105B)
Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) (CD3-HM105B)
Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) (CD3-HM105B)
Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) (CD3-HM105B)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human CD3E&CD3D, His Tag at 0.5 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 35.2 ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Asp23-Asp126 (CD3E) & Phe22-Ala105 (CD3D)
Catalog #CD3-HM105B
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Product Description
 Recombinant Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.; It contains Asp23-Asp126 (CD3E)&Phe22-Ala105 (CD3D).
Product Background
 T-cell surface glycoprotein CD3 epsilon&CD3 delta chain, also known as CD3E&CD3D, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Product Category
 Recombinant Protein
Target
 CD3E&CD3D/CD3 epsilon&CD3 delta
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Asp23-Asp126 (CD3E) & Phe22-Ala105 (CD3D)
Molecular Weight
 The protein has a predicted MW of 17.9 kDa (CD3E) and 16.7 kDa (CD3D). Due to glycosylation, the protein migrates to 25-40 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human CD3E&CD3D, His Tag at 0.5 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 35.2 ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human CD3E&CD3D, His Tag at 0.5 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 35.2 ng/ml determined by ELISA (QC Test).
Biotinylated Human CD3E&CD3D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CD3E&CD3D is greater than 95% as determined by SEC-HPLC.

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