• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human CD3E&CD3G, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 15.6ng/ml determined by ELISA. The affinity constant of 0.59 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Gln22-Asp126(CD3E) & Gln23-Ser116(CD3G)
Catalog #CD3-HM457B
Price
Regular price $66000
Regular price Sale price $66000
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-HM457B) Summary

Product Description

 Recombinant Human CD3E&CD3G Protein is expressed as a heterodimer of CD3E and CD3G by co-expression in HEK293. Subunit CD3E cotains no tag and subunit CD3G is fused with a His tag and an Avi tag at the C-terminus.It contains Gln22-Asp126 (CD3E) and Gln23-Ser116 (CD3G).

Product Background

 T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.

Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-HM457B) Specifications/Details

Protein
 CD3E&CD3G/CD3 epsilon&CD3 gamma
Synonyms
 CD3; CD3 epsilon; CD3epsilon; CD3ε; CD3 ε; CD3E; CD3e; CD3 gamma; CD3gamma; CD3γ; CD3 γ; CD3G; CD3g; CD3epsilon&CD3gamma; CD3 epsilon&CD3 gamma; CD3 epsilon & CD3 gamma; CD3ε&CD3γ; CD3 ε&CD3 γ; CD3 ε & CD3 γ; CD3E&CD3G; CD3E & CD3G; CD3 E & CD3 G; CD3e&CD3g; CD3e & CD3g; CD3 e & CD3 g;CD3 e; CD3 g; CD3 e&CD3 g
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Gln22-Asp126(CD3E) & Gln23-Ser116(CD3G)
Molecular Weight
 The protein has a predicted MW of 17.92 kDa (CD3E) and 19.67 kDa (CD3G). Due to glycosylation, the protein migrates to 25-40 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human CD3E&CD3G, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 15.6ng/ml determined by ELISA. The affinity constant of 0.59 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Biotinylated Human CD3E&CD3G, His Tag captured on CM5 Chip via SA can bind OKT3, mFc Tag with an affinity constant of 0.59 nM as determined in SPR assay (Biacore T200). Biotinylated Human CD3E&CD3G, His Tag captured on CM5 Chip via SA can bind OKT3, mFc Tag with an affinity constant of 0.59 nM as determined in SPR assay (Biacore T200).
Biotinylated Human CD3E&CD3G, His Tag captured on CM5 Chip via SA can bind Foralumab, hFc Tag with an affinity constant of 1.04 nM as determined in SPR assay (Biacore T200). Biotinylated Human CD3E&CD3G, His Tag captured on CM5 Chip via SA can bind Foralumab, hFc Tag with an affinity constant of 1.04 nM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human CD3E&CD3G, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 15.6ng/ml determined by ELISA. Immobilized Biotinylated Human CD3E&CD3G, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 15.6ng/ml determined by ELISA.
Immobilized Biotinylated Human CD3E&CD3G, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Foralumab, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. Immobilized Biotinylated Human CD3E&CD3G, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Foralumab, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
Biotinylated Human CD3E&CD3G on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human CD3E&CD3G on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CD3E&CD3G is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human CD3E&CD3G is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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