Name: Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-HM557B)
Brand: KactusBio
SKU: CD3-HM557B-100UG
Price: 660.0 USD
Availability: InStock

Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-HM557B)

Anti-CD3 Antibody, hFc Tag immobilized on CM5 Chip can bind Biotinylated Human CD3E&CD3G, hFc-Avi Tag with an affinity constant of 0.25 µM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Human CD3E&CD3G, hFc Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 30.3ng/ml determined by ELISA (QC Test).

Immobilized Anti-CD3 Anitibody, hFc Tag at 5ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human CD3E&CD3G, hFc-Avi Tag with the EC50 of 0.20ug/ml determined by ELISA.

Biotinylated CD3E&CD3G on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human CD3E&CD3G is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human CD3E&CD3G, hFc Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 30.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.25 µM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Asp23-Asp126(CD3E) & Gln23-Ser116(CD3G)

Catalog #CD3-HM557B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Asp23-Asp126(CD3E) & Gln23-Ser116(CD3G).
Product Background	T-cell surface glycoprotein CD3 epsilon&CD3 gamma chain, also known as CD3E&CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Product Category	Recombinant Protein / Antibody discovery

Protein	CD3E&CD3G/CD3 epsilon&CD3 gamma
Synonyms	CD3; CD3 epsilon; CD3epsilon; CD3ε; CD3 ε; CD3E; CD3e; CD3 gamma; CD3gamma; CD3γ; CD3 γ; CD3G; CD3g; CD3epsilon&CD3gamma; CD3 epsilon&CD3 gamma; CD3 epsilon & CD3 gamma; CD3ε&CD3γ; CD3 ε&CD3 γ; CD3 ε & CD3 γ; CD3E&CD3G; CD3E & CD3G; CD3 E & CD3 G; CD3e&CD3g; CD3e & CD3g; CD3 e & CD3 g
Accession	P07766(CD3E)&P09693(CD3G)
Species	Human
Biotinylated	yes
Amino Acid Range	Asp23-Asp126(CD3E) & Gln23-Ser116(CD3G)
Molecular Weight	The protein has a predicted MW of 40 kDa (CD3E) and 36.5 kDa (CD3G). Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human CD3E&CD3G, hFc Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 30.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.25 µM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Anti-CD3 Antibody, hFc Tag immobilized on CM5 Chip can bind Biotinylated Human CD3E&CD3G, hFc-Avi Tag with an affinity constant of 0.25 µM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human CD3E&CD3G, hFc Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 30.3ng/ml determined by ELISA (QC Test).
	Immobilized Anti-CD3 Anitibody, hFc Tag at 5ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human CD3E&CD3G, hFc-Avi Tag with the EC50 of 0.20ug/ml determined by ELISA.
	Biotinylated CD3E&CD3G on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human CD3E&CD3G is greater than 95% as determined by SEC-HPLC.