Name: Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-HM557B)
Brand: KactusBio
SKU: CD3-HM557B-100UG
Price: 660.0 USD
Availability: InStock

Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-HM557B)

Anti-CD3 Antibody, hFc Tag immobilized on CM5 Chip can bind Biotinylated Human CD3E&CD3G, hFc-Avi Tag with an affinity constant of 0.25 µM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Human CD3E&CD3G, hFc Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 30.3ng/ml determined by ELISA (QC Test).

Immobilized Anti-CD3 Anitibody, hFc Tag at 5ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human CD3E&CD3G, hFc-Avi Tag with the EC50 of 0.20ug/ml determined by ELISA.

Biotinylated CD3E&CD3G on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human CD3E&CD3G is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human CD3E&CD3G, hFc Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 30.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.25 µM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Asp23-Asp126(CD3E) & Gln23-Ser116(CD3G)

Catalog #CD3-HM557B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus of CD3E and hFc tag at the C-Terminus of CD3G.It contains Asp23-Asp126(CD3E) and Gln23-Ser116(CD3G).
Product Background	T-cell surface glycoprotein CD3 epsilon&CD3 gamma chain, also known as CD3E&CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	CD3E&CD3G/CD3 epsilon&CD3 gamma
Synonyms	CD3; CD3 epsilon; CD3epsilon; CD3ε; CD3 ε; CD3E; CD3e; CD3 gamma; CD3gamma; CD3γ; CD3 γ; CD3G; CD3g; CD3epsilon&CD3gamma; CD3 epsilon&CD3 gamma; CD3 epsilon & CD3 gamma; CD3ε&CD3γ; CD3 ε&CD3 γ; CD3 ε & CD3 γ; CD3E&CD3G; CD3E & CD3G; CD3 E & CD3 G; CD3e&CD3g; CD3e & CD3g; CD3 e & CD3 g
Accession	P07766(CD3E)&P09693(CD3G)
Species	Human
Biotinylated	yes
Amino Acid Range	Asp23-Asp126(CD3E) & Gln23-Ser116(CD3G)
Molecular Weight	The protein has a predicted MW of 40 kDa (CD3E) and 36.5 kDa (CD3G). Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human CD3E&CD3G, hFc Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 30.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.25 µM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Anti-CD3 Antibody, hFc Tag immobilized on CM5 Chip can bind Biotinylated Human CD3E&CD3G, hFc-Avi Tag with an affinity constant of 0.25 µM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human CD3E&CD3G, hFc Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 30.3ng/ml determined by ELISA (QC Test).
	Immobilized Anti-CD3 Anitibody, hFc Tag at 5ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human CD3E&CD3G, hFc-Avi Tag with the EC50 of 0.20ug/ml determined by ELISA.
	Biotinylated CD3E&CD3G on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human CD3E&CD3G is greater than 95% as determined by SEC-HPLC.