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Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein, Ultra Low Endotoxin (CD3-HM557B-UL) Summary
Product Description |
Recombinant Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus of CD3E and hFc (IgG1) tag at the C-Terminus of CD3G.It contains Asp23-Asp126(CD3E) and Gln23-Ser116(CD3G). |
Product Background |
T-cell surface glycoprotein CD3 epsilon&CD3 gamma chain, also known as CD3E&CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein, Ultra Low Endotoxin (CD3-HM557B-UL) Specifications/Details
Protein |
CD3E&CD3G/CD3 epsilon&CD3 gamma |
Synonyms |
CD3; CD3 epsilon; CD3epsilon; CD3ε; CD3 ε; CD3E; CD3e; CD3 gamma; CD3gamma; CD3γ; CD3 γ; CD3G; CD3g; CD3epsilon&CD3gamma; CD3 epsilon&CD3 gamma; CD3 epsilon & CD3 gamma; CD3ε&CD3γ; CD3 ε&CD3 γ; CD3 ε & CD3 γ; CD3E&CD3G; CD3E & CD3G; CD3 E & CD3 G; CD3e&CD3g; CD3e & CD3g; CD3 e & CD3 g |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Asp23-Asp126(CD3E) & Gln23-Ser116(CD3G) |
Molecular Weight |
The protein has a predicted MW of 40 kDa (CD3E) and 36.5 kDa (CD3G). Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human CD3E&CD3G, hFc Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 30.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.25 µM as determined in SPR assay (Biacore T200). |
Product Tag |
C-hFc (IgG1)-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Anti-CD3 Antibody, hFc Tag immobilized on CM5 Chip can bind Biotinylated Human CD3E&CD3G, hFc-Avi Tag with an affinity constant of 0.25 µM as determined in SPR assay (Biacore T200). |
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Immobilized Biotinylated Human CD3E&CD3G, hFc Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 30.3ng/ml determined by ELISA (QC Test). |
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Immobilized Anti-CD3 Anitibody, hFc Tag at 5ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human CD3E&CD3G, hFc-Avi Tag with the EC50 of 0.20ug/ml determined by ELISA. |
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Biotinylated CD3E&CD3G on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human CD3E&CD3G is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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