Biotinylated Human CD4/LEU3 Protein (CD4-HM401B)
Catalog: CD4-HM401B
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| Product Description | Recombinant Biotinylated Human CD4/LEU3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys26-Trp390. |
Product Background |
CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. |
Product Category |
Recombinant Protein |
Target |
CD4/LEU3 |
| Synonyms | CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4; CD4mut; EU3 |
Gene/Protein ID |
P01730 |
Species |
Human |
Exact Sequence |
Lys26-Trp390 |
| Molecular Weight | The protein has a predicted MW of 43.6 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Anti-CD4 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD4, His Tag with the EC50 of 12.7ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Anti-CD4 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD4, His Tag with the EC50 of 12.7ng/ml determined by ELISA. |
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The purity of Biotinylated Human CD4 is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human CD4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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