Biotinylated Human CD4/LEU3 Protein (CD4-HM401B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-CD4 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD4, His Tag with the EC50 of 12.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Lys26-Trp390

Catalog: CD4-HM401B

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Product Description Recombinant Biotinylated Human CD4/LEU3 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Lys26-Trp390.
Product Category Recombinant Protein
Target CD4/LEU3
Synonyms CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4; CD4mut; EU3
Gene/Protein ID P01730
Species Human
Exact Sequence Lys26-Trp390
Molecular Weight The protein has a predicted MW of 43.6 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-CD4 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD4, His Tag with the EC50 of 12.7ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-CD4 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD4, His Tag with the EC50 of 12.7ng/ml determined by ELISA.
The purity of Biotinylated Human CD4 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human CD4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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