Name: Biotinylated Human CD4/LEU3 Protein (CD4-HM401B)
Brand: KactusBio
SKU: CD4-HM401B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human CD4/LEU3 Protein (CD4-HM401B)

Immobilized Anti-CD4 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD4, His Tag with the EC50 of 8.4ng/ml determined by ELISA.

Biotinylated Human CD4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human CD4 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Anti-CD4 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD4, His Tag with the EC50 of 8.4ng/ml determined by ELISA.
Amino Acid Range: Lys26-Trp390

Catalog #CD4-HM401B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human CD4/LEU3 Protein (CD4-HM401B) Summary

Product Description	Recombinant Biotinylated Human CD4/LEU3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys26-Trp390.
Product Background	CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Biotinylated Human CD4/LEU3 Protein (CD4-HM401B) Specifications/Details

Protein	CD4/LEU3
Synonyms	CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4; CD4mut; EU3
Accession	P01730
Species	Human
Biotinylated	yes
Amino Acid Range	Lys26-Trp390
Molecular Weight	The protein has a predicted MW of 43.6 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Anti-CD4 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD4, His Tag with the EC50 of 8.4ng/ml determined by ELISA.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Anti-CD4 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD4, His Tag with the EC50 of 8.4ng/ml determined by ELISA.
	Biotinylated Human CD4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human CD4 is greater than 95% as determined by SEC-HPLC.