Name: Biotinylated Human CD4/LEU3 Protein (CD4-HM401B)
Brand: KactusBio
SKU: CD4-HM401B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human CD4/LEU3 Protein (CD4-HM401B)

Immobilized Anti-CD4 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD4, His Tag with the EC50 of 8.4ng/ml determined by ELISA.

Biotinylated Human CD4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human CD4 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Anti-CD4 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD4, His Tag with the EC50 of 8.4ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Lys26-Trp390

Catalog #CD4-HM401B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human CD4/LEU3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys26-Trp390.
Product Background	CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Protein	CD4/LEU3
Synonyms	CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4; CD4mut; EU3
Accession	P01730
Species	Human
Biotinylated	yes
Amino Acid Range	Lys26-Trp390
Molecular Weight	The protein has a predicted MW of 43.6 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Anti-CD4 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD4, His Tag with the EC50 of 8.4ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Anti-CD4 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD4, His Tag with the EC50 of 8.4ng/ml determined by ELISA.
	Biotinylated Human CD4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human CD4 is greater than 95% as determined by SEC-HPLC.