Biotinylated Human CD45/PTPRC Protein (PTP-HM404B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-CD45 Antibody at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD45, His Tag with the EC50 of 0.16ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln26­-Lys416

Catalog: PTP-HM404B

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Product Description Recombinant Biotinylated Human CD45/PTPRC Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln26­-Lys416.
Product Category Recombinant Protein
Target CD45/PTPRC
Synonyms L-CA; CD45; T200; PTPRC; B220; CD45R; GP180; LCA; LY5; EC 3.1.3.48
Gene/Protein ID P08575-4
Species Human
Exact Sequence Gln26­-Lys416
Molecular Weight The protein has a predicted MW of 47.3 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-CD45 Antibody at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD45, His Tag with the EC50 of 0.16ug/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-CD45 Antibody at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD45, His Tag with the EC50 of 0.16ug/ml determined by ELISA.
The purity of Biotinylated Human CD45 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human CD45 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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