Name: Biotinylated Human CD45/PTPRC Protein (PTP-HM404B)
Brand: KactusBio
SKU: PTP-HM404B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human CD45/PTPRC Protein (PTP-HM404B)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human CD45, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD45 Antibody, hFc Tag with the EC50 of 11.4ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gln26-Lys416

Catalog #PTP-HM404B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human CD45/PTPRC Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln26-Lys416.
Product Background	PTPRC (also known as CD45),T cells require the protein tyrosine phosphatase CD45 to detect and respond to antigen because it activates the Src family kinase Lck, which phosphorylates the T cell antigen receptor (TCR) complex. CD45 ativates Lck by opposing the negative regulatory kinase Csk. Paradoxically, CD45 has also been implicated in suppressing TCR signaling by dephosphorylating the same signaling motifs within the TCR complex upon which Lck acts.
Product Category	Recombinant Protein

Target	CD45/PTPRC
Synonyms	L-CA; CD45; T200; PTPRC; B220; CD45R; GP180; LCA; LY5; EC 3.1.3.48
Accession	P08575-4
Species	Human
Biotinylated	yes
Amino Acid Range	Gln26-Lys416
Molecular Weight	The protein has a predicted MW of 47.3 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human CD45, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD45 Antibody, hFc Tag with the EC50 of 11.4ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Biotinylated Human CD45, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD45 Antibody, hFc Tag with the EC50 of 11.4ng/ml determined by ELISA.
	Biotinylated Human CD45 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human CD45 is greater than 95% as determined by SEC-HPLC.

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