ELISA binding curve illustrating the interaction between biotinylated human CD5 and anti-CD5 antibody, measured by OD450 absorbance across concentration gradients.
Biotinylated Human CD5 Protein (CD5-HM501B)
Biotinylated Human CD5 Protein (CD5-HM501B)
  • Express System: HEK293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Anti-CD5 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD5, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Arg25-Asn371
Catalog #CD5-HM501B
Regular price $577.50
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Product Description

Recombinant Biotinylated Human CD5 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Arg25-Asn371.

Product Background

CD5: a type I transmembrane protein found on T cells, thymocytes, and some B cells that is a ligand for CD72 and is involved in cellular activation or adhesion; expressed in B-cell chronic lymphocytic leukemia and T-cell lymphoma.

Product Category

Recombinant Protein / Antibody discovery / CAR-T targets
Protein
CD5
Synonyms
CD5 molecule; CD5; LEU1; T1; CD5 antigen
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Arg25-Asn371
Molecular Weight
The protein has a predicted MW of 67.1 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Anti-CD5 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD5, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-hFc-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Anti-CD5 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD5, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA (QC Test). Immobilized Anti-CD5 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD5, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA (QC Test).
Biotinylated Human CD5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human CD5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CD5 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human CD5 is greater than 95% as determined by SEC-HPLC.
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