Biotinylated Human CD5 Protein (CD5-HM501B)
Biotinylated Human CD5 Protein (CD5-HM501B)
Biotinylated Human CD5 Protein (CD5-HM501B)
  • Express System: HEK293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Anti-CD5 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD5, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Arg25-Asn371
Catalog #CD5-HM501B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human CD5 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Arg25-Asn371.
Product Background
CD5: a type I transmembrane protein found on T cells, thymocytes, and some B cells that is a ligand for CD72 and is involved in cellular activation or adhesion; expressed in B-cell chronic lymphocytic leukemia and T-cell lymphoma.
Product Category
Recombinant Protein
Target
CD5
Synonyms
CD5 molecule; CD5; LEU1; T1; CD5 antigen
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Arg25-Asn371
Molecular Weight
The protein has a predicted MW of 67.1 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Anti-CD5 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD5, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
C-hFc-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-CD5 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD5, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA (QC Test).
Biotinylated Human CD5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CD5 is greater than 95% as determined by SEC-HPLC.

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