Name: Biotinylated Human CD5 Protein (CD5-HM501B)
Brand: KactusBio
SKU: CD5-HM501B-100UG
Price: 577.5 USD
Availability: InStock

ELISA binding curve illustrating the interaction between biotinylated human CD5 and anti-CD5 antibody, measured by OD450 absorbance across concentration gradients.

Biotinylated Human CD5 Protein (CD5-HM501B)

Immobilized Anti-CD5 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD5, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA (QC Test).

Biotinylated Human CD5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human CD5 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc (IgG1)-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Anti-CD5 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD5, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA (QC Test).
Amino Acid Range: Arg25-Asn371

Catalog #CD5-HM501B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human CD5 Protein (CD5-HM501B) Summary

Product Description	Recombinant Biotinylated Human CD5 Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus.It contains Arg25-Asn371.
Product Background	CD5: a type I transmembrane protein found on T cells, thymocytes, and some B cells that is a ligand for CD72 and is involved in cellular activation or adhesion; expressed in B-cell chronic lymphocytic leukemia and T-cell lymphoma.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Biotinylated Human CD5 Protein (CD5-HM501B) Specifications/Details

Protein	CD5
Synonyms	CD5 molecule; CD5; LEU1; T1; CD5 antigen
Accession	P06127
Species	Human
Biotinylated	yes
Amino Acid Range	Arg25-Asn371
Molecular Weight	The protein has a predicted MW of 67.1 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Anti-CD5 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD5, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA (QC Test).
Product Tag	C-hFc (IgG1)-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Anti-CD5 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD5, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA (QC Test).
	Biotinylated Human CD5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human CD5 is greater than 95% as determined by SEC-HPLC.