Biotinylated Human CD5 Protein, Ultra Low Endotoxin (CD5-HM401B-UL)

SKU: CD5-HM401B-UL-100UG

Biotinylated Human CD5 Protein, Ultra Low Endotoxin (CD5-HM401B-UL)

SKU: CD5-HM401B-UL-100UG
Biotinylated Human CD5 Protein, Ultra Low Endotoxin (CD5-HM401B-UL)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human CD5, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD5 Antibody, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA.
  • Amino Acid Range: Arg25-Pro372
Catalog #CD5-HM401B-UL
/
Size
image image

Order by 4pm ET for delivery on:

Biotinylated Human CD5 Protein, Ultra Low Endotoxin (CD5-HM401B-UL) Summary

Product Description

 Recombinant Biotinylated Human CD5 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Arg25-Pro372.

Product Background

 CD5: a type I transmembrane protein found on T cells, thymocytes, and some B cells that is a ligand for CD72 and is involved in cellular activation or adhesion; expressed in B-cell chronic lymphocytic leukemia and T-cell lymphoma.

Product Category

 Recombinant Protein / Antibody discovery / CAR-T targets

Biotinylated Human CD5 Protein, Ultra Low Endotoxin (CD5-HM401B-UL) Specifications/Details

Protein
 CD5
Synonyms
 CD5 molecule; CD5; LEU1; T1; CD5 antigen
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Arg25-Pro372
Molecular Weight
 The protein has a predicted MW of 41.54 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human CD5, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD5 Antibody, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA.
Product Tag
 C-His-Avi

Fluorophore

 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human CD5, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD5 Antibody, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA. Immobilized Biotinylated Human CD5, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD5 Antibody, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA.
Biotinylated Human CD5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human CD5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CD5 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human CD5 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Contact Us

support@kactusbio.us

Recommended Products

Loading...
Recently viewed