Name: Biotinylated Human CD58 Protein (CD5-HM418B)
Brand: KactusBio
SKU: CD5-HM418B-100UG
Price: 578.0 USD
Availability: InStock

Biotinylated Human CD58 Protein (CD5-HM418B)

Immobilized Biotinylated Human CD58, His Avi Tag at 1ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD2, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA.

Biotinylated Human CD58 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human CD58 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human CD58, His Avi Tag at 1ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD2, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA.
Amino Acid Range: Phe29-Arg215

Catalog #CD5-HM418B

Size

100UG

500UG

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Biotinylated Human CD58 Protein (CD5-HM418B) Summary

Product Description	Recombinant Biotinylated Human CD58 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Phe29-Arg215.
Product Background	Loss of CD58 is a common mechanism for tumor immune evasion in lymphoid malignancies. CD58 loss is known to occur due to both genetic and non-genetic causes; therefore, we hypothesized that restoring CD58 expression in lymphoma cells may be an effective treatment approach. EZH2 is involved in the epigenetic silencing of CD58 in lymphoma cells as a mechanism for tumor immune escape, and EZH2 inhibitors are able to restore epigenetically suppressed CD58 expression.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery/Others

Biotinylated Human CD58 Protein (CD5-HM418B) Specifications/Details

Protein	CD58
Synonyms	Lymphocyte function-associated antigen 3;Ag3;CD58;LFA3
Accession	AAH05930
Species	Human
Biotinylated	no
Amino Acid Range	Phe29-Arg215
Molecular Weight	The protein has a predicted MW of 24.37 kDa. Due to glycosylation, the protein migrates to 35-58 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human CD58, His Avi Tag at 1ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD2, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human CD58, His Avi Tag at 1ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD2, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA.
	Biotinylated Human CD58 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human CD58 is greater than 95% as determined by SEC-HPLC.