Biotinylated Human CD7 Protein (CD7-HM401B)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Biotinylated: yes
Activity: Immobilized Biotinylated Human CD7, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD7 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ala26-Pro180

Catalog: CD7-HM401B

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Product Description	Recombinant Biotinylated Human CD7 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala26-Pro180.
Product Background	CD7, also known as Leu-9, is an approximately 40 kDa glycosylated and palmitoylated transmembrane protein in the immunoglobulin superfamily.CD7 is expressed on T cells, NK cells , myeloid progenitor cells, and CD19 B progenitor cells. Among CD8 T cells, the CD7-bright population preferentially contains naïve and memory cells, while more weak expressors are primarily effector cells.
Product Category	Recombinant Protein

Target	CD7
Synonyms	CD7 molecule; CD7; LEU-9; Tp40; TP41; GP40
Gene/Protein ID	P09564
Species	Human
Exact Sequence	Ala26-Pro180
Molecular Weight	The protein has a predicted MW of 19.3 kDa. Due to glycosylation, the protein migrates to 34-48 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Biotinylated Human CD7, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD7 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Biotinylated Human CD7, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD7 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA.
	The purity of Biotinylated Human CD7 is greater than 95% as determined by SEC-HPLC.
	Biotinylated Human CD7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.