Biotinylated Human CD7 Protein (CD7-HM401B)
Biotinylated Human CD7 Protein (CD7-HM401B)
Biotinylated Human CD7 Protein (CD7-HM401B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human CD7, His Tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Anti-CD7 Antibody, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ala26-Pro180
Catalog #CD7-HM401B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human CD7 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala26-Pro180.
Product Background
CD7, also known as Leu-9, is an approximately 40 kDa glycosylated and palmitoylated transmembrane protein in the immunoglobulin superfamily. CD7 is expressed on T cells, NK cells, myeloid progenitor cells, and CD19 B progenitor cells. Among CD8 T cells, the CD7-bright population preferentially contains naïve and memory cells, while more weak expressors are primarily effector cells.
Product Category
Recombinant Protein
Target
CD7
Synonyms
CD7 molecule; CD7; LEU-9; Tp40; TP41; GP40
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Ala26-Pro180
Molecular Weight
The protein has a predicted MW of 19.3 kDa. Due to glycosylation, the protein migrates to 34-48 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human CD7, His Tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Anti-CD7 Antibody, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human CD7, His Tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Anti-CD7 Antibody, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA (QC Test).
Biotinylated Human CD7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CD7 is greater than 95% as determined by SEC-HPLC.

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