Product Description |
Recombinant Biotinylated Human CD7 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala26-Pro180. |
Product Background |
CD7, also known as Leu-9, is an approximately 40 kDa glycosylated and palmitoylated transmembrane protein in the immunoglobulin superfamily. CD7 is expressed on T cells, NK cells, myeloid progenitor cells, and CD19 B progenitor cells. Among CD8 T cells, the CD7-bright population preferentially contains naïve and memory cells, while more weak expressors are primarily effector cells. |
Product Category |
Recombinant Protein |
Target |
CD7 |
Synonyms |
CD7 molecule; CD7; LEU-9; Tp40; TP41; GP40 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ala26-Pro180 |
Molecular Weight |
The protein has a predicted MW of 19.3 kDa. Due to glycosylation, the protein migrates to 34-48 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human CD7, His Tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Anti-CD7 Antibody, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Biotinylated Human CD7, His Tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Anti-CD7 Antibody, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA (QC Test). |
|
|
Biotinylated Human CD7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human CD7 is greater than 95% as determined by SEC-HPLC. |
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