• Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Biotinylated Human CD74, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD74 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.
  • Amino Acid Range: Gln73-Met232
Catalog #CD7-HM175B
Price
Regular price $578.00
Regular price Sale price $578.00
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human CD74/DHLAG Protein (Primary Amine Labeling) (CD7-HM175B) Summary

Product Description

 Recombinant Biotinylated Human CD74/DHLAG Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the N-terminus.It contains Gln73-Met232.

Product Background

 CD74 (invariant MHC class II) regulates protein trafficking and is a receptor for macrophage migration inhibitory factor (MIF) and d-dopachrome tautomerase (d-DT/MIF-2). CD74 expression is increased in tubular cells and/or glomerular podocytes and parietal cells in human metabolic nephropathies, polycystic kidney disease, graft rejection and kidney cancer and in experimental diabetic nephropathy and glomerulonephritis.

Product Category

 Recombinant Protein / Antibody discovery / ADC targets

Biotinylated Human CD74/DHLAG Protein (Primary Amine Labeling) (CD7-HM175B) Specifications/Details

Protein
 CD74/DHLAG
Synonyms
 CD74; Ii; DHLAG; HLADG; Ia-GAMMA; p33; CLIP; INVG34; Ia GAMMA ; II
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Gln73-Met232
Molecular Weight
 The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human CD74, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD74 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.
Product Tag
 N-His

Fluorophore

 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human CD74, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD74 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. Immobilized Biotinylated Human CD74, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD74 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.
Biotinylated Human CD74 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human CD74 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...
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