Biotinylated Human CD74/DHLAG Protein (Primary Amine Labeling) (CD7-HM175B)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Biotinylated Human CD74, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD74 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.
  • Amino Acid Range: Gln73-Met232
  • Other Available Proteins:
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Catalog #CD7-HM175B
Regular price $578.00
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Biotinylated Human CD74/DHLAG Protein (Primary Amine Labeling) (CD7-HM175B) Summary

Product Description

Recombinant Biotinylated Human CD74/DHLAG Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the N-terminus.It contains Gln73-Met232.

Product Background

CD74 (invariant MHC class II) regulates protein trafficking and is a receptor for macrophage migration inhibitory factor (MIF) and d-dopachrome tautomerase (d-DT/MIF-2). CD74 expression is increased in tubular cells and/or glomerular podocytes and parietal cells in human metabolic nephropathies, polycystic kidney disease, graft rejection and kidney cancer and in experimental diabetic nephropathy and glomerulonephritis.

Product Category

Recombinant Protein / Antibody discovery / ADC targets

Biotinylated Human CD74/DHLAG Protein (Primary Amine Labeling) (CD7-HM175B) Specifications/Details

Protein
CD74/DHLAG
Synonyms
CD74; Ii; DHLAG; HLADG; Ia-GAMMA; p33; CLIP; INVG34; Ia GAMMA ; II
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Gln73-Met232
Molecular Weight
The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human CD74, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD74 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.
Product Tag
N-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human CD74, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD74 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. Immobilized Biotinylated Human CD74, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD74 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.
Biotinylated Human CD74 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human CD74 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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