Name: Biotinylated Human CD8 alpha&beta Heterodimer Protein (CD8-HM4ABB)
Brand: KactusBio
SKU: CD8-HM4ABB-100UG
Price: 660.0 USD
Availability: InStock

Biotinylated Human CD8 alpha&beta Heterodimer Protein (CD8-HM4ABB)

Immobilized Biotinylated Human CD8 alpha&beta, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD8 alpha&beta Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA.

Biotinylated Human CD8 alpha&beta Heterodimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human CD8 alpha&beta Heterodimer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi, C-Flag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human CD8 alpha&beta, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD8 alpha&beta Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA.
Amino Acid Range: Ser22-Asp182(CD8 alpha) acidic tail & Leu22-Pro170(CD8 beta) basic tail
Other Available Proteins:

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Catalog #CD8-HM4ABB

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human CD8 alpha&beta Heterodimer Protein (CD8-HM4ABB) Summary

Product Description	Recombinant Biotinylated Human CD8 alpha&beta Heterodimer Protein is expressed as a heterodimer of CD8 alpha and CD8 beta by co-expression in HEK293. Subunit CD8 alpha is fused with a His tag and a Avi tag at the C-terminus and subunit CD8 beta is fused with a Flag tag at the C-terminus.It contains Ser22-Asp182 (CD8 alpha) acidic tail and Leu22-Pro170 (CD8 beta) basic tail.
Product Background	CD8 alpha&beta (CD8αβ) is a heterodimeric form of CD8. CD8α is required for surface expression of CD8β. The extracellular IgV-like domain of CD8α interacts with the α3 portion of the class I MHC molecule. CD8αβ is expressed on human peripheral T cells and functions as a coreceptor and can greatly increase the sensitivity and breadth of antigen recognition by CD8⁺ peripheral T cells bearing TCR.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery/Others

Biotinylated Human CD8 alpha&beta Heterodimer Protein (CD8-HM4ABB) Specifications/Details

Protein	CD8 alpha&beta
Synonyms	CD8 alphaβ CD8A&CD8B;
Accession	P01732-1(CD8 alpha)&P10966-1(CD8 beta)
Species	Human
Biotinylated	yes
Amino Acid Range	Ser22-Asp182(CD8 alpha) acidic tail & Leu22-Pro170(CD8 beta) basic tail
Molecular Weight	The protein has a predicted MW of 25.35 kDa (CD8 alpha) and 22.62 kDa (CD8 beta). Due to glycosylation, the protein migrates to 32-36 kDa and 38-45 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human CD8 alpha&beta, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD8 alpha&beta Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA.
Product Tag	C-His-Avi, C-Flag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human CD8 alpha&beta, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD8 alpha&beta Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA.
	Immobilized Biotinylated Human CD8 alpha&beta, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA.
	Immobilized Biotinylated Human CD8 alpha&beta, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD8 beta Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA.
	Biotinylated Human CD8 alpha&beta Heterodimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human CD8 alpha&beta Heterodimer is greater than 95% as determined by SEC-HPLC.