Biotinylated Human CD8 alpha/CD8A Protein (CD8-HM48AB)
Biotinylated Human CD8 alpha/CD8A Protein (CD8-HM48AB)
Biotinylated Human CD8 alpha/CD8A Protein (CD8-HM48AB)
Biotinylated Human CD8 alpha/CD8A Protein (CD8-HM48AB)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Biotinylated Human CD8 alpha, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ser22-Asp182
Catalog #CD8-HM48AB
Regular price $525.00
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Product Description
Recombinant Biotinylated Human CD8 alpha/CD8A Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser22-Asp182.
Product Background
CD8 alpha enhances the responses of antigen-specific CTL activated through TCR through binding MHC class I, favoring lipid raft partitioning of TCR, and inducing intracellular signaling. CD8 alpha is also found on dendritic cells and rat macrophages, but whether CD8 alpha enhances responses of a partner receptor, like TCR, to activate these cells is not known.
Product Category
Recombinant Protein
Target
CD8 alpha/CD8A
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Ser22-Asp182
Molecular Weight
The protein has a predicted MW of 20.5 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human CD8 alpha, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human CD8 alpha, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test).
Biotinylated Human CD8 alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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