Name: Biotinylated Human CD8 alpha/CD8A Protein (CD8-HM48AB)
Brand: KactusBio
SKU: CD8-HM48AB-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human CD8 alpha/CD8A Protein (CD8-HM48AB)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Biotinylated Human CD8 alpha, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Ser22-Asp182

Catalog #CD8-HM48AB

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human CD8 alpha/CD8A Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser22-Asp182.
Product Background	CD8 alpha enhances the responses of antigen-specific CTL activated through TCR through binding MHC class I, favoring lipid raft partitioning of TCR, and inducing intracellular signaling. CD8 alpha is also found on dendritic cells and rat macrophages, but whether CD8 alpha enhances responses of a partner receptor, like TCR, to activate these cells is not known.
Product Category	Recombinant Protein

Target	CD8 alpha/CD8A
Accession	P01732-1
Species	Human
Biotinylated	yes
Amino Acid Range	Ser22-Asp182
Molecular Weight	The protein has a predicted MW of 20.5 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human CD8 alpha, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Biotinylated Human CD8 alpha, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test).
	Biotinylated Human CD8 alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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