Name: Biotinylated Human CD81 Nanodisc (CD8-HM18NB)
Brand: KactusBio
SKU: CD8-HM18NB-100UG
Price: 2166.0 USD
Availability: InStock

Biotinylated Human CD81 Nanodisc (CD8-HM18NB)

Biotinylated Human CD81 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-CD81 Antibody, hFc Tag with an affinity constant of 2.38 nM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Human CD81 Nanodisc, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD81 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA.

Express System: HEK293
Product Tag: C-His
Activity: Immobilized Biotinylated Human CD81 Nanodisc, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD81 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. The affinity constant of 2.38 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Met1-Tyr236

Catalog #CD8-HM18NB

Size

100UG

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Biotinylated Human CD81 Nanodisc (CD8-HM18NB) Summary

Product Description	Recombinant Biotinylated Human CD81 Nanodisc is expressed from HEK293 with His tag at the C-terminus.It contains Met1-Tyr236.
Product Background	CD81 (TAPA-1) is a member of the tetraspanin family, a large group of evolutionary conserved membrane proteins containing 4 transmembrane domains. The most distinctive feature of the tetraspanin proteins is the ability to form lateral associations with one another and to cluster dynamically with multiple cell surface receptors. Tetraspanins are capable of recruiting specific intracellular signaling enzymes, including protein kinase C, that is known to be involved in integrin phosphorylation.
Product Category	Recombinant Protein / Antibody discovery / Multiple transmembrane proteins / Nanodisc

Biotinylated Human CD81 Nanodisc (CD8-HM18NB) Specifications/Details

Protein	CD81
Synonyms	TAPA-1; Tspan-28; TAPA1; TSPAN28; CVID6; S5.7
Accession	P60033
Species	Human
Biotinylated	yes
Amino Acid Range	Met1-Tyr236
Molecular Weight	The protein has a predicted MW of 38.9 kDa.
Activity	Immobilized Biotinylated Human CD81 Nanodisc, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD81 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. The affinity constant of 2.38 nM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Expression System	HEK293
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Stability And Storage	Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Biotinylated Human CD81 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-CD81 Antibody, hFc Tag with an affinity constant of 2.38 nM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human CD81 Nanodisc, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD81 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA.