Biotinylated Human CD93/C1q R1 Protein (CD9-HM493B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human CD93, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IGFBP7, hFc Tag with the EC50 of 25.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.17 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Thr22-Lys580
Catalog #CD9-HM493B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human CD93/C1q R1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Thr22-Lys580.
Product Background
CD93 has been shown critical roles in inflammatory and immune diseases. CD93 silencing increased IL-6 and TSLP, but not IL-33 levels in culture supernatants. HDM-induced asthma mice showed significant airway hyperresponsiveness and inflammation with Th2 cytokine activation, along with decreased CD93 expression in bronchial epithelial cells and lung homogenates but increased serum CD93 levels.
Product Category
Recombinant Protein
Target
CD93/C1q R1
Synonyms
C1q/MBL/SPA receptor; C1qR; C1qR(p); C1qRp; CDw93; CD93; C1QR1; MXRA4; ECSM3; dJ737E23.1
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Thr22-Lys580
Molecular Weight
The protein has a predicted MW of 61.2 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human CD93, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IGFBP7, hFc Tag with the EC50 of 25.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.17 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human CD93, His-Avi Tag immobilized on CM5 Chip can bind Human IGFBP-7, His Tag with an affinity constant of 0.17 uM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human CD93, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IGFBP7, hFc Tag with the EC50 of 25.9ng/ml determined by ELISA (QC Test).
Biotinylated Human CD93 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CD93 is greater than 95% as determined by SEC-HPLC.

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