Biotinylated Human CD93/C1q R1 Protein (CD9-HM493B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human CD93, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IGFBP7, hFc Tag with the EC50 of 27.9ng/ml determined by ELISA. The affinity constant of 0.17 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Thr22-Lys580

Catalog: CD9-HM493B

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Product Description Recombinant Biotinylated Human CD93/C1q R1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Thr22-Lys580.
Product Category Recombinant Protein
Target CD93/C1q R1
Synonyms C1q/MBL/SPA receptor; C1qR; C1qR(p); C1qRp; CDw93; CD93; C1QR1; MXRA4; ECSM3; dJ737E23.1
Gene/Protein ID Q9NPY3
Species Human
Exact Sequence Thr22-Lys580
Molecular Weight The protein has a predicted MW of 61.2 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Human CD93, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IGFBP7, hFc Tag with the EC50 of 27.9ng/ml determined by ELISA. The affinity constant of 0.17 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human CD93, His-Avi Tag immobilized on CM5 Chip can bind Human IGFBP-7, His Tag with an affinity constant of 0.17 uM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human CD93, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IGFBP7, hFc Tag with the EC50 of 27.9ng/ml determined by ELISA.
The purity of Biotinylated Human CD93 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human CD93 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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