Name: Biotinylated Human CD93/C1q R1 Protein (CD9-HM493B)
Brand: KactusBio
SKU: CD9-HM493B-100UG
Price: 577.5 USD
Availability: InStock

SPR sensorgram illustrating concentration-dependent binding responses for kinetic profiling of molecular interactions.

Biotinylated Human CD93, His-Avi Tag immobilized on CM5 Chip can bind Human IGFBP-7, His Tag with an affinity constant of 0.17 uM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Human CD93, His-Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IGFBP7, hFc Tag with the EC50 of 29.8ng/ml determined by ELISA (QC Test).

Biotinylated Human CD93 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human CD93 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human CD93, His-Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IGFBP7, hFc Tag with the EC50 of 29.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.17 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Thr22-Lys580

Catalog #CD9-HM493B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human CD93/C1q R1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Thr22-Lys580.
Product Background	CD93 has been shown critical roles in inflammatory and immune diseases. CD93 silencing increased IL-6 and TSLP, but not IL-33 levels in culture supernatants. HDM-induced asthma mice showed significant airway hyperresponsiveness and inflammation with Th2 cytokine activation, along with decreased CD93 expression in bronchial epithelial cells and lung homogenates but increased serum CD93 levels.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	CD93/C1q R1
Synonyms	C1q/MBL/SPA receptor; C1qR; C1qR(p); C1qRp; CDw93; CD93; C1QR1; MXRA4; ECSM3; dJ737E23.1
Accession	Q9NPY3
Species	Human
Biotinylated	yes
Amino Acid Range	Thr22-Lys580
Molecular Weight	The protein has a predicted MW of 61.2 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human CD93, His-Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IGFBP7, hFc Tag with the EC50 of 29.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.17 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Biotinylated Human CD93, His-Avi Tag immobilized on CM5 Chip can bind Human IGFBP-7, His Tag with an affinity constant of 0.17 uM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human CD93, His-Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IGFBP7, hFc Tag with the EC50 of 29.8ng/ml determined by ELISA (QC Test).
	Biotinylated Human CD93 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human CD93 is greater than 95% as determined by SEC-HPLC.

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