Biotinylated Human CD98 Protein (CD9-HM498B)
|Recombinant Biotinylated Human CD98 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains Arg206-Ala630.
|The type II transmembrane protein CD98, best known as the heavy chain of the heterodimeric amino acid transporters (HAT), is required for the surface expression and basolateral localization of this transporter complex in polarized epithelial cells. CD98 also interacts with beta1 integrins resulting in an increase in their affinity for ligand. In this study we explored the role of the transmembrane and cytoplasmic domains of CD98 on integrin-dependent cell adhesion and migration in polarized renal epithelial cells.
|The protein has a predicted MW of 49.75 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
|Immobilized Biotinylated Human CD98, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for HAnti-CD98 Antibody, hFc Tag with the EC50 of 9.7ng/ml determined by ELISA. See testing image for detail.
|> 95% as determined by Tris-Bis PAGE
|Less than 1EU per ug by the LAL method.
|Shipped with dry ice.
|Supplied as 0.22 um filtered solution in PBS (pH 7.4).
|Stability And Storage
|Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.