Name: Biotinylated Human CD98 Protein (CD9-HM498B)
Brand: KactusBio
SKU: CD9-HM498B-100UG
Price: 577.5 USD
Availability: InStock

ELISA binding curve of biotinylated human CD98 His-tag protein showing OD450 response to increasing concentrations of anti-CD98 hFc-tagged antibody.

Biotinylated Human CD98 Protein (CD9-HM498B)

Immobilized Biotinylated Human CD98, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for HAnti-CD98 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA.

Biotinylated Human CD98 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human CD98 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human CD98, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for HAnti-CD98 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Arg206-Ala630

Catalog #CD9-HM498B

Size

100UG

500UG

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Biotinylated Human CD98 Protein (CD9-HM498B) Summary

Product Description	Recombinant Biotinylated Human CD98 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains Arg206-Ala630.
Product Background	The type II transmembrane protein CD98, best known as the heavy chain of the heterodimeric amino acid transporters (HAT), is required for the surface expression and basolateral localization of this transporter complex in polarized epithelial cells. CD98 also interacts with beta1 integrins resulting in an increase in their affinity for ligand. In this study we explored the role of the transmembrane and cytoplasmic domains of CD98 on integrin-dependent cell adhesion and migration in polarized renal epithelial cells.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human CD98 Protein (CD9-HM498B) Specifications/Details

Protein	CD98
Synonyms	4F2hc; CD98; SLC3A2; 4F2; CD98HC; MDU1; NACAE; 4T2HC
Accession	AAH01061
Species	Human
Biotinylated	yes
Amino Acid Range	Arg206-Ala630
Molecular Weight	The protein has a predicted MW of 49.75 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human CD98, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for HAnti-CD98 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human CD98, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for HAnti-CD98 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA.
	Biotinylated Human CD98 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human CD98 is greater than 95% as determined by SEC-HPLC.