• Express System: HEK293
  • Product Tag: C-hFc (IgG1)-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: The affinity constant of 6.88 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Lys369-Thr667
Catalog #CDC-HM50CB
Price
Regular price $66000
Regular price Sale price $66000
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human CDCP1 CTF Protein (CDC-HM50CB) Summary

Product Description

 Recombinant Biotinylated Human CDCP1 CTF Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-terminus.It contains Lys369-Thr667.

Product Background

 Tumor metastasis depends on the dynamic regulation of cell adhesion through β1-integrin. The Cub-Domain Containing Protein-1, CDCP1, is a transmembrane glycoprotein which regulates cell adhesion. Overexpression and loss of CDCP1 have been observed in the same cancer types to promote metastatic progression.

Biotinylated Human CDCP1 CTF Protein (CDC-HM50CB) Specifications/Details

Protein
 CDCP1
Synonyms
 CDCP1; CD318; SIMA135; TRASK; UNQ2486; PRO5773
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Lys369-Thr667
Molecular Weight
 The protein has a predicted MW of 61.66 kDa. Due to glycosylation, the protein migrates to 85-85 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 6.88 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-hFc (IgG1)-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Biotinylated Human CDCP1 CTF, hFc-Avi Tag captured on CM5 Chip via Streptavidin can bind Anti-CDCP1 CTF Antibody with an affinity constant of 6.88 nM as determined in SPR assay (Biacore T200). Biotinylated Human CDCP1 CTF, hFc-Avi Tag captured on CM5 Chip via Streptavidin can bind Anti-CDCP1 CTF Antibody with an affinity constant of 6.88 nM as determined in SPR assay (Biacore T200).
Biotinylated Human CDCP1 CTF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human CDCP1 CTF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CDCP1 CTF is greater than 90% as determined by SEC-HPLC. The purity of Biotinylated Human CDCP1 CTF is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...