Biotinylated Human CDCP1 Protein (CDC-HM401B)
Biotinylated Human CDCP1 Protein (CDC-HM401B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human CDCP1, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Phe30-Leu666
Catalog #CDC-HM401B
Regular price $577.50
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Size
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Biotinylated Human CDCP1 Protein (CDC-HM401B) Summary

Product Description

Recombinant Biotinylated Human CDCP1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe30-Leu666.

Product Background

Tumor metastasis depends on the dynamic regulation of cell adhesion through β1-integrin. The Cub-Domain Containing Protein-1, CDCP1, is a transmembrane glycoprotein which regulates cell adhesion. Overexpression and loss of CDCP1 have been observed in the same cancer types to promote metastatic progression.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Biotinylated Human CDCP1 Protein (CDC-HM401B) Specifications/Details

Protein
CDCP1
Synonyms
CDCP1; CD318; SIMA135; TRASK; UNQ2486; PRO5773
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Phe30-Leu666
Molecular Weight
The protein has a predicted MW of 74.6 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human CDCP1, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS, 100mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human CDCP1, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA. Immobilized Biotinylated Human CDCP1, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA.
Biotinylated Human CDCP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human CDCP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Anti-CDCP1 Antibody, hFc-Avi Tag on ProA-Biosensor can bind Biotinylated Human CDCP1, His-Avi Tag with an affinity constant of 3.97 nM as determined in BLI assay (Gator® Prime). Loaded Anti-CDCP1 Antibody, hFc-Avi Tag on ProA-Biosensor can bind Biotinylated Human CDCP1, His-Avi Tag with an affinity constant of 3.97 nM as determined in BLI assay (Gator® Prime).
The purity of Biotinylated Human CDCP1 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human CDCP1 is greater than 95% as determined by SEC-HPLC.
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