Biotinylated Human CDCP1 Protein (CDC-HM401B)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Biotinylated: yes
Activity: Immobilized Biotinylated Human CDCP1, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Phe30-Leu666

Catalog: CDC-HM401B

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Product Description	Recombinant Biotinylated Human CDCP1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe30-Leu666.
Product Background	Tumor metastasis depends on the dynamic regulation of cell adhesion through β1-integrin. The Cub-Domain Containing Protein-1, CDCP1, is a transmembrane glycoprotein which regulates cell adhesion. Overexpression and loss of CDCP1 have been observed in the same cancer types to promote metastatic progression.
Product Category	Recombinant Protein

Target	CDCP1
Synonyms	CDCP1; CD318; SIMA135; TRASK; UNQ2486; PRO5773
Gene/Protein ID	Q9H5V8-1
Species	Human
Exact Sequence	Phe30-Leu666
Molecular Weight	The protein has a predicted MW of 74.6 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Biotinylated Human CDCP1, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Biotinylated Human CDCP1, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA.
	The purity of Biotinylated Human CDCP1 is greater than 95% as determined by SEC-HPLC.
	Biotinylated Human CDCP1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.