Biotinylated Human CDH6/Cadherin 6 Protein (CDH-HM406B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: >95% as determined by Bis-Tris PAGE;>90% as determined by HPLC
  • Activity: Immobilized Biotinylated Human CDH6, His-Avi Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CDH6 Antibody, Rabbit IgG Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Thr19-Ala615
Catalog #CDH-HM406B
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Product Description
 Recombinant Biotinylated Human CDH6/Cadherin 6 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Thr19-Ala615.
Product Background
 Cadherin 6 (CDH6) is an adhesion molecule localizing to the endometrial luminal epithelial cell surface in the mid-secretory/receptive phase and knockdown of CDH6 in the Ishikawa cells (receptive endometrial epithelial cell line) compromises cell integrity.
Product Category
 Recombinant Protein
Target
 CDH6/Cadherin 6
Synonyms
 Cadherin-6; Cadherin6; K-cadherin; CDH6; CAD6; KCAD; Kidney cadherin
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Thr19-Ala615
Molecular Weight
 The protein has a predicted MW of 69.29 kDa. Due to glycosylation, the protein migrates to 75-90 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human CDH6, His-Avi Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CDH6 Antibody, Rabbit IgG Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 >95% as determined by Bis-Tris PAGE;>90% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human CDH6, His-Avi Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CDH6 Antibody, Rabbit IgG Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test).
Biotinylated Human CDH6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CDH6 is greater than 90% as determined by SEC-HPLC.

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