Biotinylated Human CEACAM-8/CD66b Protein (CAM-HM408B)
Biotinylated Human CEACAM-8/CD66b Protein (CAM-HM408B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 6.13 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gln35-Ser319
Catalog #CAM-HM408B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human CEACAM-8/CD66b Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln35-Ser319.
Product Background
Eosinophils and their products are likely important in the pathophysiology of allergic diseases, such as bronchial asthma, and in host immunity to parasitic organisms. CD66b (CEACAM8, CGM6, NCA-95) is a single chain, GPI-anchored, highly glycosylated protein belonging to the carcinoembryonic Ag supergene family. CD66b is an activation marker for human granulocytes.
Product Category
Recombinant Protein
Target
CEACAM-8/CD66b
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Gln35-Ser319
Molecular Weight
The protein has a predicted MW of 34.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 6.13 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human CEACAM-8, His Tag immobilized on CM5 Chip can bind Human CEACAM-6, His Tag with an affinity constant of 6.13 uM as determined in SPR assay (Biacore T200).
Biotinylated Human CEACAM-8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CEACAM-8 is greater than 95% as determined by SEC-HPLC.

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