• Express System: HEK293
  • Product Tag: C-His
  • Activity: Immobilized Biotinylated Human Claudin 1 Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Claudin 1 Antibody, hFc Tag with the EC50 of 15.9ng/ml determined by ELISA. The affinity constant of 99.98 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Met1-Val211
Catalog #CLD-HM1N1B
Price
Regular price $2,16600
Regular price Sale price $2,16600
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Size
Avantor VWR Fisher Scientific

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Biotinylated Human Claudin 1 Nanodisc (CLD-HM1N1B) Summary

Product Description

 Recombinant Biotinylated Human Claudin 1 Nanodisc is expressed from HEK293 with His tag at the C-terminus.It contains Met1-Val211.

Product Background

 Claudin-1 (CLDN1), a major component of tight junction complexes in the epithelium, maintains cellular polarity, and plays a critical role in cell-to-cell communication as well as epithelial cell homeostasis. Moreover, CLDN1 expression is up-regulated in several cancers, and the level of CLDN1 expression has been proposed as a prognostic marker of patient survival.

Biotinylated Human Claudin 1 Nanodisc (CLD-HM1N1B) Specifications/Details

Protein
 Claudin 1
Synonyms
 Claudin-1; CLDN1; CLD1; SEMP1
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Met1-Val211
Molecular Weight
 The protein has a predicted MW of 35.69 kDa.
Activity
 Immobilized Biotinylated Human Claudin 1 Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Claudin 1 Antibody, hFc Tag with the EC50 of 15.9ng/ml determined by ELISA. The affinity constant of 99.98 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Stability And Storage
 Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Biotinylated Claudin 1 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-Claudin 1 Antibody, hFc Tag with an affinity constant of 99.98 nM as determined in SPR assay (Biacore T200). Biotinylated Claudin 1 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-Claudin 1 Antibody, hFc Tag with an affinity constant of 99.98 nM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human Claudin 1 Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Claudin 1 Antibody, hFc Tag with the EC50 of 15.9ng/ml determined by ELISA. Immobilized Biotinylated Human Claudin 1 Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Claudin 1 Antibody, hFc Tag with the EC50 of 15.9ng/ml determined by ELISA.
Other Available Proteins:
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