Biotinylated Human Claudin 18.2 Protein-VLP (CLD-HE1822B)

  • Express System: Expi293
  • Purity: > 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human Claudin 18.2 VLP on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-Claudin18.2 Antibody, hFc Tag with the EC50 of 62ng/ml determined by ELISA. The affinity constant of 7.74 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Met1-Val261

Catalog: CLD-HE1822B

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Product Description Recombinant Biotinylated Full length Human Claudin 18.2 Protein-VLP is expressed from Expi293.It contains Met1-Val261.
Product Category Recombinant Protein
Target Claudin 18.2
Synonyms CLDN18; CLDN18.2; Claudin-18.2; Claudin-18; DKFZp564B2062; SFTA5; SFTPJ
Gene/Protein ID P56856-2
Species Human
Exact Sequence Met1-Val261
Molecular Weight The protein has a predicted MW of 29 kDa.
Activity Immobilized Biotinylated Human Claudin 18.2 VLP on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-Claudin18.2 Antibody, hFc Tag with the EC50 of 62ng/ml determined by ELISA. The affinity constant of 7.74 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Express System Expi293
Purity > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.Notice: If you need it for immunization, Do Not use any adjuvant.
Reconstitution Centrifuge tubes before opening.Redissolve with distilled water to 100μl.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Amine coupling SA on CM5 Chip, SA capture Biotinylated Human Claudin 18.2 VLP, Anti-Claudin18.2 Ab. as analyte; The affinity constant of 7.74 nM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human Claudin 18.2 VLP on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-Claudin18.2 Antibody, hFc Tag with the EC50 of 62ng/ml determined by ELISA.
The purity of Biotinylated Human Claudin 18.2 VLP is greater than 95% as determined by SEC-HPLC.

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