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Biotinylated Human Claudin 3 Nanodisc (CLD-HM13NB) Summary
Product Description |
Recombinant Biotinylated Human Claudin 3 Nanodisc is expressed from HEK293 with His tag at the C-terminus.It contains Met1-Val220. |
Product Background |
Claudin-3 is a memember of the Claudin family, which is present in many epithelia including skin, lung, kidney and intestine. It beautifully localizes on the cell-to-cell junctions of MCF-7 cells, clearly forming a continuous belt-like structure surrounding the cells. It has often been associated with the progression and metastasis of various tumors. |
Product Category |
Recombinant Protein / Antibody discovery / Multiple transmembrane proteins / Nanodisc |
Biotinylated Human Claudin 3 Nanodisc (CLD-HM13NB) Specifications/Details
Protein |
Claudin 3 |
Synonyms |
Claudin-3; CPE-R 2; CPE-receptor 2; hRVP1; CLDN3; C7orf1; CPETR2; RVP1 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Met1-Val220 |
Molecular Weight |
The protein has a predicted MW of 36.4 kDa. |
Activity |
Immobilized Biotinylated Human Claudin 3, His Tag at 2 ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Claudin 3 Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. The affinity constant of 24.48 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4). |
Stability And Storage |
Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Biotinylated Human Claudin 3 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-Claudin 3 Antibody, hFc Tag with an affinity constant of 24.48 nM as determined in SPR assay (Biacore T200). |
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Immobilized Biotinylated Human Claudin 3, His Tag at 2 ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Claudin 3 Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. |
Other Available Proteins:
Finding available proteins...
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