• Express System: HEK293
  • Purity: > 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human Claudin 6 VLP at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Claudin6 Antibody, mFc Tag with the EC50 of 6.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.46 nM as determined in SPR assay (Biacore T200).
  • Negative Control: Biotinylated VLP Control (GPR-HM05CB)
  • Amino Acid Range: Met1-Val220
Catalog #CLD-HM006B
Price
Regular price $1,17600
Regular price Sale price $1,17600
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human Claudin 6 VLP (CLD-HM006B) Summary

Product Description

 Recombinant Biotinylated Full length Human Claudin 6 VLP is expressed from HEK293.It contains Met1-Val220 (It may have cross reaction with anti-His antibody).

Product Background

 Claudin-6 is a multipass transmembrane protein in the Claudin family. Claudin-6 is expressed by epithelial cells where it participates in tissue development and the maintenance of tight junction integrity. Human Claudin-6 shares 88% and 86% amino acid sequence identity with mouse and rat Claudin-6, respectively.

Biotinylated Human Claudin 6 VLP (CLD-HM006B) Specifications/Details

Protein
 Claudin 6
Synonyms
 Claudin-6; Skullin; CLDN6; Claudin6; Skullin 2
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Met1-Val220
Molecular Weight
 The target protein has a predicted MW of 24.5 kDa.
Activity
 Immobilized Biotinylated Human Claudin 6 VLP at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Claudin6 Antibody, mFc Tag with the EC50 of 6.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.46 nM as determined in SPR assay (Biacore T200).
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22um filtered solution in PBS, 300mM L-arginine (pH 7.4).
Stability And Storage
 Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Biotinylated Human Claudin 6 VLP captured on CM5 Chip via streptavidin can bind Anti-Claudin 6 Antibody with an affinity constant of 0.46 nM as determined in SPR assay (Biacore T200). Biotinylated Human Claudin 6 VLP captured on CM5 Chip via streptavidin can bind Anti-Claudin 6 Antibody with an affinity constant of 0.46 nM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human Claudin 6 VLP at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Claudin6 Antibody, mFc Tag with the EC50 of 6.5ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human Claudin 6 VLP at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Claudin6 Antibody, mFc Tag with the EC50 of 6.5ng/ml determined by ELISA (QC Test).
Loaded Biotinylated Human Claudin 6 VLP on Streptavidin-Biosensor can bind Anti-Claudin 6 Antibody, hFc Tag with an affinity constant of 1.46 nM as determined in BLI assay . Loaded Biotinylated Human Claudin 6 VLP on Streptavidin-Biosensor can bind Anti-Claudin 6 Antibody, hFc Tag with an affinity constant of 1.46 nM as determined in BLI assay .
The purity of Biotinylated Human Claudin 6 VLP is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human Claudin 6 VLP is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...