Product Description |
Recombinant Biotinylated Human CLEC12A/MICL/CLL-1 Protein is expressed from HEK293 with His tag at the N-Terminus and Avi tag at the C-Terminus.It contains His65-Ala265. |
Product Background |
CLEC12A has recently been identified as an antigen, expressed on leukemic stem cells and leukemic blasts. Given the fact that this expression profile seems stable throughout diagnosis, treatment and relapse on leukemic blasts and leukemic stem cells, CLEC12A can be considered a highly potent and reliable marker for the detection of measurable residual disease and therefore applicable for risk stratification and prognostication in AML. |
Product Category |
Recombinant Protein |
Target |
CLEC12A/MICL/CLL-1 |
Synonyms |
MICL; CLL-1; CLEC12A; CLL1; DCAL2; DCAL-2; CD371; CD303; CLECSF11; CLECSF7; DLEC; HECL; PRO34150; DCAL-2 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
His65-Ala265 |
Molecular Weight |
The protein has a predicted MW of 26.4 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human CLEC12A, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CLEC12A Antibody, hFc Tag with the EC50 of 29.6ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His,C-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Biotinylated Human CLEC12A, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CLEC12A Antibody, hFc Tag with the EC50 of 29.6ng/ml determined by ELISA. |
|
|
Biotinylated Human CLEC12A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Biotinylated Human CLEC12A is greater than 95% as determined by SEC-HPLC. |
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