Biotinylated Human CLEC12A/MICL/CLL-1 Protein (CLE-HM42AB)

  • Express System: HEK293
  • Product Tag: N-His,C-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-CLEC12A Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CLEC12A, His Tag with the EC50 of 0.41ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: His65-Ala265

Catalog: CLE-HM42AB

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Product Description Recombinant Biotinylated Human CLEC12A/MICL/CLL-1 Protein is expressed from HEK293 with His tag at the N-Terminus and Avi tag at the C-Terminus.It contains His65-Ala265.

Product Background

CLEC12A has recently been identified as an antigen, expressed on leukemic stem cells and leukemic blasts. Given the fact that this expression profile seems stable throughout diagnosis, treatment and relapse on leukemic blasts and leukemic stem cells, CLEC12A can be considered a highly potent and reliable marker for the detection of measurable residual disease and therefore applicable for risk stratification and prognostication in AML.

Product Category

Recombinant Protein

Target

CLEC12A/MICL/CLL-1
Synonyms MICL; CLL-1; CLEC12A; CLL1; DCAL2; DCAL-2; CD371; CD303; CLECSF11; CLECSF7; DLEC; HECL; PRO34150; DCAL-2

Gene/Protein ID

Q5QGZ9-2

Species

Human

Exact Sequence

His65-Ala265
Molecular Weight The protein has a predicted MW of 26.4 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Anti-CLEC12A Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CLEC12A, His Tag with the EC50 of 0.41ug/ml determined by ELISA. See testing image for detail.

Product Tag

N-His,C-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-CLEC12A Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CLEC12A, His Tag with the EC50 of 0.41ug/ml determined by ELISA.
The purity of Biotinylated Human CLEC12A is greater than 95% as determined by SEC-HPLC.
Biotinylated Human CLEC12A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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