Biotinylated Human CLEC9A Protein (Primary Amine Labeling) (CLE-HM29AB)
Biotinylated Human CLEC9A Protein (Primary Amine Labeling) (CLE-HM29AB)
  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Anti-CLEC9A Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CLEC9A, hFc Tag with the EC50 of 30.9ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Lys57-Val241
Catalog #CLE-HM29AB
Regular price $525.00
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Product Description

Recombinant Biotinylated Human CLEC9A Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the N-terminus.It contains Lys57-Val241.

Product Background

CLEC9A expression was significantly higher in psoriatic skin compared with healthy donor. In psoriatic skin and PsA ST, CLEC9A() cells were in close proximity to TUNEL() cells. SF CLEC9A levels were significantly lower compared with paired PsA serum. Adalimumab treatment did not affect CLEC9A serum level and skin expression. The downregulation of synovial CLEC9A might be associated with a novel mechanism by which anti-TNF therapy might reduce CD8-mediated inflammation in PsA patients.

Product Category

Recombinant Protein / Antibody discovery

Protein

CLEC9A

Synonyms

CD370; CLEC9A; 9830005G06Rik; DNGR-1; HEEE9341; UNQ9341

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Lys57-Val241

Molecular Weight

The protein has a predicted MW of 48.5 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Anti-CLEC9A Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CLEC9A, hFc Tag with the EC50 of 30.9ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

N-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS, 100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Anti-CLEC9A Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CLEC9A, hFc Tag with the EC50 of 30.9ng/ml determined by ELISA (QC Test). Immobilized Anti-CLEC9A Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CLEC9A, hFc Tag with the EC50 of 30.9ng/ml determined by ELISA (QC Test).
Biotinylated Human CLEC9A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human CLEC9A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CLEC9A is greater than 90% as determined by SEC-HPLC. The purity of Biotinylated Human CLEC9A is greater than 90% as determined by SEC-HPLC.

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