Biotinylated Human CLEC9A Protein (Primary Amine Labeling) (CLE-HM29AB)
  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Anti-CLEC9A Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CLEC9A, hFc Tag with the EC50 of 17.3ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Lys57-Val241
Catalog #CLE-HM29AB
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Product Description
 Recombinant Biotinylated Human CLEC9A Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the N-terminus.It contains Lys57-Val241.
Product Background
 CLEC9A expression was significantly higher in psoriatic skin compared with healthy donor. In psoriatic skin and PsA ST, CLEC9A() cells were in close proximity to TUNEL() cells. SF CLEC9A levels were significantly lower compared with paired PsA serum. Adalimumab treatment did not affect CLEC9A serum level and skin expression. The downregulation of synovial CLEC9A might be associated with a novel mechanism by which anti-TNF therapy might reduce CD8-mediated inflammation in PsA patients.
Product Category
 Recombinant Protein
Target
 CLEC9A
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Lys57-Val241
Molecular Weight
 The protein has a predicted MW of 48.5 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Anti-CLEC9A Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CLEC9A, hFc Tag with the EC50 of 17.3ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
 N-hFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS, 100mM L-arginine (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Anti-CLEC9A Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CLEC9A, hFc Tag with the EC50 of 17.3ng/ml determined by ELISA (QC Test).
Biotinylated Human CLEC9A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CLEC9A is greater than 90% as determined by SEC-HPLC.

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