Name: Biotinylated Human CLEC9A Protein (Primary Amine Labeling) (CLE-HM29AB)
Brand: KactusBio
SKU: CLE-HM29AB-100UG
Price: 577.5 USD
Availability: InStock

ELISA binding curve showing the dose-dependent interaction between biotinylated Human CLEC9A, hFc Tag, and Anti-CLEC9A Antibody, hFc Tag. The plot presents mean absorbance (OD450) versus the log concentration of biotinylated Human CLEC9A (µg/ml), demonstrating a sigmoidal response.

Biotinylated Human CLEC9A Protein (Primary Amine Labeling) (CLE-HM29AB)

Immobilized Anti-CLEC9A Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CLEC9A, hFc Tag with the EC50 of 30.9ng/ml determined by ELISA (QC Test).

Biotinylated Human CLEC9A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human CLEC9A is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Anti-CLEC9A Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CLEC9A, hFc Tag with the EC50 of 30.9ng/ml determined by ELISA (QC Test).
Amino Acid Range: Lys57-Val241

Catalog #CLE-HM29AB

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human CLEC9A Protein (Primary Amine Labeling) (CLE-HM29AB) Summary

Product Description	Recombinant Biotinylated Human CLEC9A Protein (Primary Amine Labeling) is expressed from HEK293 with hFc (IgG1) tag at the N-terminus.It contains Lys57-Val241.
Product Background	CLEC9A is a recently discovered C-type lectin receptor involved in sensing necrotic cells. In humans, this receptor is selectively expressed by BDCA3(+) myeloid dendritic cells (mDCs), which have been proposed to be the main human cross-presenting mDCs and may represent the human homologue of murine CD8(+) DCs. In mice, it was demonstrated that antigens delivered with antibodies to CLEC9A are presented by CD8(+) DCs to both CD4(+) and CD8(+) T cells and induce antitumor immunity in a melanoma model.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human CLEC9A Protein (Primary Amine Labeling) (CLE-HM29AB) Specifications/Details

Protein	CLEC9A
Synonyms	CD370; CLEC9A; 9830005G06Rik; DNGR-1; HEEE9341; UNQ9341
Accession	Q6UXN8
Species	Human
Biotinylated	yes
Amino Acid Range	Lys57-Val241
Molecular Weight	The protein has a predicted MW of 48.5 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Anti-CLEC9A Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CLEC9A, hFc Tag with the EC50 of 30.9ng/ml determined by ELISA (QC Test).
Product Tag	N-hFc (IgG1)
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS, 100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Anti-CLEC9A Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CLEC9A, hFc Tag with the EC50 of 30.9ng/ml determined by ELISA (QC Test).
	Biotinylated Human CLEC9A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human CLEC9A is greater than 90% as determined by SEC-HPLC.