Biotinylated Human Coagulation factor III/Tissue Factor Protein (CF3-HM401B)
Biotinylated Human Coagulation factor III/Tissue Factor Protein (CF3-HM401B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human Coagulation Factor III, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Tissue Factor Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ser33-Glu251
Catalog #CF3-HM401B
Regular price $578.00
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Product Description

Recombinant Biotinylated Human Coagulation factor III/Tissue Factor Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ser33-Glu251.

Product Background

Tissue factor (also named Coagulation Factor III) is a cell surface glycoprotein responsible for initiating the extrinsic pathway of coagulation.Tissue factor is the primary cellular initiator of blood coagulation via interaction with coagulation factor VII. Aberrant expression of tissue factor is responsible for thrombosis during septic shock, atherosclerosis and cancer.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

Coagulation factor III/Tissue Factor

Synonyms

Tissue factor; Coagulation Factor III; TF; Thromboplastin; CD142; F3; FLJ17960; TFA

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Ser33-Glu251

Molecular Weight

The protein has a predicted MW of 27.7 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Human Coagulation Factor III, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Tissue Factor Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human Coagulation Factor III, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Tissue Factor Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. Immobilized Biotinylated Human Coagulation Factor III, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Tissue Factor Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.
Biotinylated Human Coagulation factor III on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human Coagulation factor III on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Coagulation factor III is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human Coagulation factor III is greater than 95% as determined by SEC-HPLC.

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