- Express System: HEK293
- Product Tag: C-His-Avi
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Biotinylated Human Coagulation Factor III, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Tissue Factor Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Ser33-Glu251
Product Description |
Recombinant Biotinylated Human Coagulation factor III/Tissue Factor Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ser33-Glu251. |
Product Background |
Tissue factor (also named Coagulation Factor III) is a cell surface glycoprotein responsible for initiating the extrinsic pathway of coagulation.Tissue factor is the primary cellular initiator of blood coagulation via interaction with coagulation factor VII. Aberrant expression of tissue factor is responsible for thrombosis during septic shock, atherosclerosis and cancer. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
Coagulation factor III/Tissue Factor |
Synonyms |
Tissue factor; Coagulation Factor III; TF; Thromboplastin; CD142; F3; FLJ17960; TFA |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ser33-Glu251 |
Molecular Weight |
The protein has a predicted MW of 27.7 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human Coagulation Factor III, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Tissue Factor Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in PBS (pH 7.4). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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