Name: Biotinylated Human Coagulation factor III/Tissue Factor Protein, Ultra Low Endotoxin (CF3-HM401B-UL)
Brand: KactusBio
SKU: CF3-HM401B-UL-100UG
Price: 578.0 USD
Availability: InStock

Biotinylated Human Coagulation factor III/Tissue Factor Protein, Ultra Low Endotoxin (CF3-HM401B-UL)

Immobilized Biotinylated Human Coagulation Factor III, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Tissue Factor Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.

Biotinylated Human Coagulation factor III on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human Coagulation factor III is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human Coagulation Factor III, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Tissue Factor Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. See testing image for detail.Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate Boc-VPR-AMC. The AC50 is < 4µg/mL.
Amino Acid Range: Ser33-Glu251

Catalog #CF3-HM401B-UL

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human Coagulation factor III/Tissue Factor Protein, Ultra Low Endotoxin (CF3-HM401B-UL) Summary

Product Description	Recombinant Biotinylated Human Coagulation factor III/Tissue Factor Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ser33-Glu251.
Product Background	Tissue factor (also named Coagulation Factor III) is a cell surface glycoprotein responsible for initiating the extrinsic pathway of coagulation.Tissue factor is the primary cellular initiator of blood coagulation via interaction with coagulation factor VII. Aberrant expression of tissue factor is responsible for thrombosis during septic shock, atherosclerosis and cancer.
Product Category	Recombinant Protein / Antibody discovery / ADC targets

Biotinylated Human Coagulation factor III/Tissue Factor Protein, Ultra Low Endotoxin (CF3-HM401B-UL) Specifications/Details

Protein	Coagulation factor III/Tissue Factor
Synonyms	Tissue factor; Coagulation Factor III; TF; Thromboplastin; CD142; F3; FLJ17960; TFA
Accession	P13726-1
Species	Human
Biotinylated	yes
Amino Acid Range	Ser33-Glu251
Molecular Weight	The protein has a predicted MW of 27.7 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human Coagulation Factor III, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Tissue Factor Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. See testing image for detail.Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate Boc-VPR-AMC. The AC50 is < 4µg/mL.
Product Tag	C-His-Avi
Fluorophore	Biotin-labeled
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human Coagulation Factor III, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Tissue Factor Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.
	Biotinylated Human Coagulation factor III on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human Coagulation factor III is greater than 95% as determined by SEC-HPLC.

Other Available Proteins:

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Biotinylated Human Coagulation factor III/Tissue Factor Protein, Ultra Low Endotoxin (CF3-HM401B-UL)

Biotinylated Human Coagulation factor III/Tissue Factor Protein, Ultra Low Endotoxin (CF3-HM401B-UL)

Biotinylated Human Coagulation factor III/Tissue Factor Protein, Ultra Low Endotoxin (CF3-HM401B-UL) Summary

Product Description

Product Background

Product Category

Biotinylated Human Coagulation factor III/Tissue Factor Protein, Ultra Low Endotoxin (CF3-HM401B-UL) Specifications/Details

Fluorophore

Shipping, Reconstitution, & Storage

Validation Images

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