Biotinylated Human Coagulation factor XI Protein (pro form) (FXI-HM401

Immobilized Biotinylated Human Coagulation Factor XI, His Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-factor XI Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA.

Biotinylated Human Coagulation factor XI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human Coagulation factor XI is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human Coagulation Factor XI, His Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-factor XI Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate, t-butyloxycarbonyl-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin (Boc-IEGR-AMC). The specific activity is >100 pmoles/min/ug.
Amino Acid Range: Glu19-Val625

Catalog #FXI-HM401B

Price

$578⁰⁰

Size

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Biotinylated Human Coagulation factor XI Protein (pro form) (FXI-HM401B) Summary

Product Description

Recombinant Biotinylated Human Coagulation factor XI Protein (pro form) is expressed from HEK293 with His tag and Avi tag at the C-terminus. The protein needs to be activated by Thermolysin for an activated form.It contains Glu19-Val625.

Product Background

Coagulation factor (F)XI has been described as a component of the early phase of the contact pathway of blood coagulation, acting downstream of factor XII.In contrast to its mild hemostatic activities, FXI is known to play a significant role in thrombosis, as it is a demonstrated independent risk factor for deep vein thrombosis, ischemic stroke, and myocardial infarction. A better understanding of the varied and complex role of FXI in both thrombosis and hemostasis will help to allow better prediction of bleeding risk in FXI-deficient patients and also informing the development of targeted agents to inhibit the thrombotic activities of FXI while preserving hemostasis.

Biotinylated Human Coagulation factor XI Protein (pro form) (FXI-HM401B) Specifications/Details

Protein	Coagulation factor XI
Synonyms	Coagulation factor XI; FXI; PTA; F11;
Accession	P03951-1
Species	Human
Biotinylated	yes
Amino Acid Range	Glu19-Val625
Molecular Weight	The protein has a predicted MW of 70.93 kDa. Due to glycosylation, the protein migrates to 75-95 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human Coagulation Factor XI, His Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-factor XI Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate, t-butyloxycarbonyl-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin (Boc-IEGR-AMC). The specific activity is >100 pmoles/min/ug.
Product Tag	C-His-Avi
Fluorophore	Biotin-labeled
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 25mM HEPES, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human Coagulation Factor XI, His Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-factor XI Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA.
	Biotinylated Human Coagulation factor XI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human Coagulation factor XI is greater than 95% as determined by SEC-HPLC.