Biotinylated Human CTGF/CCN2 Protein (CGF-HM401B)
|Recombinant Biotinylated Human CTGF/CCN2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln27-Ala349.
|Connective tissue growth factor (CTGF) is a member of the CCN matricellular protein family, consisting of four domains, that regulates the signaling of other growth factors and promotes kidney fibrosis.CTGF can simultaneously interact with several factors with its four domains. The microenvironment differs depending on the types of cells and tissues and differentiation stages of these cells.
|IGFBP8; IBP-8; CCN2; NOV2; HCS24; CTGF; CTGRP; Fisp12; MGC102839
|The protein has a predicted MW of 38.4 kDa. Due to glycosylation, the protein migrates to 43-53 kDa based on Tris-Bis PAGE result.
|Immobilized Biotinylated Human CTGF, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CTGF Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. See testing image for detail.
|> 95% as determined by Tris-Bis PAGE
|Less than 1EU per ug by the LAL method.
|Shipped at ambient temperature.
|Lyophilized from 0.22um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
|Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
|Stability And Storage
|Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.