Product Description |
Recombinant Biotinylated Human CXCL4 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Glu32-Ser101. |
Product Background |
Chemokines regulate leukocyte migration during physiological and pathological conditions. It is currently accepted that these chemotactic cytokines are also important in the development and progression of cancer. CXCL4 and its non-allelic variant CXCL4L1 are two platelet-associated chemokines that have been attributed anti-tumoral activity as a result of their angiostatic potential and the chemotactic activity for anti-tumoral leukocytes. |
Product Category |
Recombinant Protein |
Target |
CXCL4 |
Synonyms |
PF-4; Oncostatin-A; CXCL4; SCYB4; Iroplact; MGC138298 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Glu32-Ser101 |
Molecular Weight |
The protein has a predicted MW of 10.68 kDa. The protein migrates to 13 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CCL5, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CXCL4, His Tag with the EC50 of 1.1ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His-Avi |
Express System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 4mM HCl (pH 3.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 4mM HCl (pH 3.0). |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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