Biotinylated Human CXCL4 Protein (CXC-HM4L4B)

Express System: E.coli
Product Tag: N-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE
Biotinylated: yes
Activity: Immobilized Human CCL5, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CXCL4, His Tag with the EC50 of 1.1ug/ml determined by ELISA. See testing image for detail.
Exact Sequence: Glu32-Ser101

Catalog: CXC-HM4L4B

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Product Description	Recombinant Biotinylated Human CXCL4 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Glu32-Ser101.
Product Background	Chemokines regulate leukocyte migration during physiological and pathological conditions. It is currently accepted that these chemotactic cytokines are also important in the development and progression of cancer. CXCL4 and its non-allelic variant CXCL4L1 are two platelet-associated chemokines that have been attributed anti-tumoral activity as a result of their angiostatic potential and the chemotactic activity for anti-tumoral leukocytes.
Product Category	Recombinant Protein

Target	CXCL4
Synonyms	PF-4; Oncostatin-A; CXCL4; SCYB4; Iroplact; MGC138298
Gene/Protein ID	NP_002610.1
Species	Human
Exact Sequence	Glu32-Ser101
Molecular Weight	The protein has a predicted MW of 10.68 kDa. The protein migrates to 13 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CCL5, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CXCL4, His Tag with the EC50 of 1.1ug/ml determined by ELISA. See testing image for detail.
Product Tag	N-His-Avi
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 4mM HCl (pH 3.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 4mM HCl (pH 3.0).
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human CCL5, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CXCL4, His Tag with the EC50 of 1.1ug/ml determined by ELISA.
	Biotinylated Human CXCL4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.