Name: Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein (CD8-HM428B)
Brand: KactusBio
SKU: CD8-HM428B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein (CD8-HM428B)

Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His-Avi Tag with an affinity constant of 41.59 uM as determined in SPR assay (Biacore T200).

Immobilized Anti-CD28 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His Tag with the EC50 of 24.4ng/ml determined by ELISA (QC Test).

Biotinylated Human/Cynomolgus/Rhesus macaque CD28 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Anti-CD28 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His Tag with the EC50 of 24.4ng/ml determined by ELISA (QC Test). The affinity constant of 41.59 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Asn19-Pro152

Catalog #CD8-HM428B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Asn19-Pro152.
Product Background	CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	CD28
Synonyms	CD28 antigen (Tp44); CD28 molecule; CD28; MGC138290
Accession	P10747
Species	Human/Cynomolgus/Rhesus macaque
Biotinylated	yes
Amino Acid Range	Asn19-Pro152
Molecular Weight	The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 38-55 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Anti-CD28 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His Tag with the EC50 of 24.4ng/ml determined by ELISA (QC Test). The affinity constant of 41.59 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His-Avi Tag with an affinity constant of 41.59 uM as determined in SPR assay (Biacore T200).
	Immobilized Anti-CD28 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His Tag with the EC50 of 24.4ng/ml determined by ELISA (QC Test).
	Biotinylated Human/Cynomolgus/Rhesus macaque CD28 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.