Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein (CD8-HM428B)
Catalog: CD8-HM428B
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Product Description | Recombinant Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal.It contains Asn19-Pro152. |
Product Category | Recombinant Protein |
Target | CD28 |
Synonyms | CD28 antigen (Tp44); CD28 molecule; CD28; MGC138290 |
Gene/Protein ID | P10747 |
Species | Human/Cynomolgus/Rhesus macaque |
Exact Sequence | Asn19-Pro152 |
Molecular Weight | The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Anti-CD28 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His Tag with the EC50 of 24.4ng/ml determined by ELISA. See testing image for detail. |
Product Tag | N-His-Avi |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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