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Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein (CD8-HM428B) Summary
Product Description |
Recombinant Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Asn19-Pro152. |
Product Background |
CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein (CD8-HM428B) Specifications/Details
Protein |
CD28 |
Synonyms |
CD28 antigen (Tp44); CD28 molecule; CD28; MGC138290 |
Accession |
|
Species |
Human/Cynomolgus/Rhesus macaque |
Biotinylated |
yes |
Amino Acid Range |
Asn19-Pro152 |
Molecular Weight |
The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 38-55 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His-Avi Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA. The affinity constant of 41.59 uM as determined in SPR assay (Biacore T200). |
Product Tag |
N-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His-Avi Tag with an affinity constant of 41.59 uM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His-Avi Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA. |
|
|
Biotinylated Human/Cynomolgus/Rhesus macaque CD28 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
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