Product Description |
Recombinant Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Asn19-Pro152. |
Product Background |
CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. |
Product Category |
Recombinant Protein |
Target |
CD28 |
Synonyms |
CD28 antigen (Tp44); CD28 molecule; CD28; MGC138290 |
Accession |
|
Species |
Human/Cynomolgus/Rhesus macaque |
Biotinylated |
yes |
Amino Acid Range |
Asn19-Pro152 |
Molecular Weight |
The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 38-55 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Anti-CD28 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His Tag with the EC50 of 24.4ng/ml determined by ELISA (QC Test). The affinity constant of 41.59 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
N-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His-Avi Tag with an affinity constant of 41.59 uM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Anti-CD28 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His Tag with the EC50 of 24.4ng/ml determined by ELISA (QC Test). |
|
|
Biotinylated Human/Cynomolgus/Rhesus macaque CD28 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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