Biotinylated Human DDR1 Protein, Ultra Low Endotoxin (DDR-HM4R1B-UL)

SKU: DDR-HM4R1B-UL-100UG

Biotinylated Human DDR1 Protein, Ultra Low Endotoxin (DDR-HM4R1B-UL)

SKU: DDR-HM4R1B-UL-100UG
Biotinylated Human DDR1 Protein, Ultra Low Endotoxin (DDR-HM4R1B-UL)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human DDR1, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-DDR1 Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA.
  • Amino Acid Range: Asp21-Ala417
Catalog #DDR-HM4R1B-UL
Regular price $578.00
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Biotinylated Human DDR1 Protein, Ultra Low Endotoxin (DDR-HM4R1B-UL) Summary

Product Description

Recombinant Biotinylated Human DDR1 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Asp21-Ala417.

Product Background

Discoidin domain receptor1 (DDR1) is a collagen activated receptor tyrosine kinase and an attractive anti-fibrotic target. Its expression is mainly limited to epithelial cells located in several organs including skin, kidney, liver and lung. DDR1's biology is elusive, with unknown downstream activation pathways; however, it may act as a mediator of the stromal-epithelial interaction, potentially controlling the activation state of the resident quiescent fibroblasts.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery/Others

Biotinylated Human DDR1 Protein, Ultra Low Endotoxin (DDR-HM4R1B-UL) Specifications/Details

Protein
DDR1
Synonyms
HGK2; MCK-10; CAK; EDDR1; NEP; NTRK4; PTK3A; RTK6; TRKE; CD167a; EC 2.7.10.1
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Asp21-Ala417
Molecular Weight
The protein has a predicted MW of 46.93 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human DDR1, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-DDR1 Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA.
Product Tag
C-His-Avi

Fluorophore

Biotin-labeled
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human DDR1, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-DDR1 Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA. Immobilized Biotinylated Human DDR1, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-DDR1 Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA.
Biotinylated Human DDR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human DDR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human DDR1 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human DDR1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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