Biotinylated Human DKK1 C terminal Domain Protein (DKK-HM51CB)

Immobilized Human LRP-6, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated DKK1 C terminal Domain, hFc Tag with the EC50 of 2.07ug/ml determined by ELISA (QC Test).

Immobilized Anti-DKK1 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human DKK1 C terminal Domain, hFc Tag with the EC50 of 19.7ng/ml determined by ELISA (QC Test).

Biotinylated Human DKK1 C terminal Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Express System: HEK293
Product Tag: C-hFc-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human LRP-6, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated DKK1 C terminal Domain, hFc Tag with the EC50 of 2.07ug/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Met178-His266

Catalog #DKK-HM51CB

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human DKK1 C terminal Domain Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Met178-His266.
Product Background	Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes.
Product Category	Recombinant Protein / Antibody discovery

Protein	DKK1 C terminal Domain
Synonyms	Dickkopf-related 1; Dickkopf-1; Dkk-1; Dkk1; hDkk-1; hDkk1; SK
Accession	O94907
Species	Human
Biotinylated	yes
Amino Acid Range	Met178-His266
Molecular Weight	The protein has a predicted MW of 38.69 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human LRP-6, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated DKK1 C terminal Domain, hFc Tag with the EC50 of 2.07ug/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-hFc-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM NaAc, 150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc, 150mM NaCl (pH 5.0).
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human LRP-6, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated DKK1 C terminal Domain, hFc Tag with the EC50 of 2.07ug/ml determined by ELISA (QC Test).
	Immobilized Anti-DKK1 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human DKK1 C terminal Domain, hFc Tag with the EC50 of 19.7ng/ml determined by ELISA (QC Test).
	Biotinylated Human DKK1 C terminal Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.