Name: Biotinylated Human DKK1 C terminal Domain Protein (DKK-HM51CB)
Brand: KactusBio
SKU: DKK-HM51CB-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human DKK1 C terminal Domain Protein (DKK-HM51CB)

Immobilized Human LRP-6, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated DKK1 C terminal Domain, hFc Tag with the EC50 of 2.07ug/ml determined by ELISA (QC Test).

Immobilized Anti-DKK1 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human DKK1 C terminal Domain, hFc Tag with the EC50 of 19.7ng/ml determined by ELISA (QC Test).

Biotinylated Human DKK1 C terminal Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Express System: HEK293
Product Tag: C-hFc (IgG1)-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human LRP-6, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated DKK1 C terminal Domain, hFc Tag with the EC50 of 2.07ug/ml determined by ELISA (QC Test).
Amino Acid Range: Met178-His266

Catalog #DKK-HM51CB

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human DKK1 C terminal Domain Protein (DKK-HM51CB) Summary

Product Description	Recombinant Biotinylated Human DKK1 C terminal Domain Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus.It contains Met178-His266.
Product Background	Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human DKK1 C terminal Domain Protein (DKK-HM51CB) Specifications/Details

Protein	DKK1
Synonyms	Dickkopf-related 1; Dickkopf-1; Dkk-1; Dkk1; hDkk-1; hDkk1; SK
Accession	O94907
Species	Human
Biotinylated	yes
Amino Acid Range	Met178-His266
Molecular Weight	The protein has a predicted MW of 38.69 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human LRP-6, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated DKK1 C terminal Domain, hFc Tag with the EC50 of 2.07ug/ml determined by ELISA (QC Test).
Product Tag	C-hFc (IgG1)-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM NaAc, 150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in 20mM NaAc, 150mM NaCl (pH 5.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human LRP-6, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated DKK1 C terminal Domain, hFc Tag with the EC50 of 2.07ug/ml determined by ELISA (QC Test).
	Immobilized Anti-DKK1 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human DKK1 C terminal Domain, hFc Tag with the EC50 of 19.7ng/ml determined by ELISA (QC Test).
	Biotinylated Human DKK1 C terminal Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.