Biotinylated Human DKK1 C terminal Domain Protein (DKK-HM51CB)
Biotinylated Human DKK1 C terminal Domain Protein (DKK-HM51CB)
Biotinylated Human DKK1 C terminal Domain Protein (DKK-HM51CB)
  • Express System: HEK293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human LRP-6, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated DKK1 C terminal Domain, hFc Tag with the EC50 of 2.07ug/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Met178-His266
Catalog #DKK-HM51CB
Regular price $577.50
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Product Description

Recombinant Biotinylated Human DKK1 C terminal Domain Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Met178-His266.

Product Background

Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others
Protein
DKK1
Synonyms
Dickkopf-related 1; Dickkopf-1; Dkk-1; Dkk1; hDkk-1; hDkk1; SK
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Met178-His266
Molecular Weight
The protein has a predicted MW of 38.69 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human LRP-6, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated DKK1 C terminal Domain, hFc Tag with the EC50 of 2.07ug/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-hFc-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 20mM NaAc, 150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in 20mM NaAc, 150mM NaCl (pH 5.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human LRP-6, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated DKK1 C terminal Domain, hFc Tag with the EC50 of 2.07ug/ml determined by ELISA (QC Test). Immobilized Human LRP-6, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated DKK1 C terminal Domain, hFc Tag with the EC50 of 2.07ug/ml determined by ELISA (QC Test).
Immobilized Anti-DKK1 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human DKK1 C terminal Domain, hFc Tag with the EC50 of 19.7ng/ml determined by ELISA (QC Test). Immobilized Anti-DKK1 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human DKK1 C terminal Domain, hFc Tag with the EC50 of 19.7ng/ml determined by ELISA (QC Test).
Biotinylated Human DKK1 C terminal Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human DKK1 C terminal Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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