Biotinylated Human DKK1 N terminal Domain Protein (DKK-HM50NB) Summary
Product Description |
Recombinant Biotinylated Human DKK1 N terminal Domain Protein is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus.It contains Thr32-Asp142. |
Product Background |
Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Biotinylated Human DKK1 N terminal Domain Protein (DKK-HM50NB) Specifications/Details
Protein |
DKK1 |
Synonyms |
Dickkopf-related 1; Dickkopf-1; Dkk-1; Dkk1; hDkk-1; hDkk1; SK |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Thr32-Asp142 |
Molecular Weight |
The protein has a predicted MW of 40.25 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-hFc (IgG1)-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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