Product Description |
Recombinant Biotinylated Human DKK1 N terminal Domain Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Thr32-Asp142. |
Product Background |
Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes. |
Product Category |
Recombinant Protein |
Target |
DKK1 N terminal Domain |
Synonyms |
Dickkopf-related 1; Dickkopf-1; Dkk-1; Dkk1; hDkk-1; hDkk1; SK |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Thr32-Asp142 |
Molecular Weight |
The protein has a predicted MW of 40.25 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-hFc-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Biotinylated Human DKK1 N terminal Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Biotinylated Human DKK1 N terminal Domain is greater than 95% as determined by SEC-HPLC. |
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