Biotinylated Human DKK1 Protein (DKK-HM401B)
Biotinylated Human DKK1 Protein (DKK-HM401B)
Biotinylated Human DKK1 Protein (DKK-HM401B)
Biotinylated Human DKK1 Protein (DKK-HM401B)
Biotinylated Human DKK1 Protein (DKK-HM401B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Anti-DKK1 Antibody, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human DKK1, His Tag with the EC50 of 3.1ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Thr32-His266
Catalog #DKK-HM401B
Regular price $525.00
/
Size

Order now, ships by:

Product Description
Recombinant Biotinylated Human DKK1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Thr32-His266.
Product Background
Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Dkk antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation.
Product Category
Recombinant Protein
Target
DKK1
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Thr32-His266
Molecular Weight
The protein has a predicted MW of 28.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Anti-DKK1 Antibody, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human DKK1, His Tag with the EC50 of 3.1ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-DKK1 Antibody, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human DKK1, His Tag with the EC50 of 3.1ng/ml determined by ELISA.
Immobilized Biotinylated Human DKK1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-5, hFc Tag with the EC50 of 66.8ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human DKK1, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 0.34ug/ml determined by ELISA.
Biotinylated Human DKK1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human DKK1 is greater than 95% as determined by SEC-HPLC.

Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Contact Us

support@kactusbio.us

Recently viewed