Biotinylated Human DKK1 Protein (DKK-HM401B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human DKK1, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 3.2ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Thr32-His266

Catalog: DKK-HM401B

$657.00
Size:

Order now get it on

Product Description Recombinant Biotinylated Human DKK1 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Thr32-His266.
Product Category Recombinant Protein
Target DKK1
Gene/Protein ID O94907
Species Human
Exact Sequence Thr32-His266
Molecular Weight The protein has a predicted MW of 28.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Human DKK1, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 3.2ug/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human DKK1, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 3.2ug/ml determined by ELISA.
Immobilized Anti-DKK1 Antibody, hFc Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human DKK1, His Tag with the EC50 of 2.3ng/ml determined by ELISA.
The purity of Biotinylated Human DKK1 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human DKK1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

You may also be interested in