Name: Biotinylated Human DKK1 Protein (DKK-HM401B)
Brand: KactusBio
SKU: DKK-HM401B-100UG
Price: 577.5 USD
Availability: InStock

ELISA binding curve of biotinylated human DKK1 His-tag showing interaction with human LRP-6 mFc-tag protein.

Biotinylated Human DKK1 Protein (DKK-HM401B)

Immobilized Biotinylated Human DKK1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-5, hFc Tag with the EC50 of 66.8ng/ml determined by ELISA (QC Test).

Immobilized Biotinylated Human DKK1, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 0.34ug/ml determined by ELISA.

Immobilized Anti-DKK1 Antibody, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human DKK1, His Tag with the EC50 of 3.1ng/ml determined by ELISA.

Biotinylated Human DKK1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human DKK1 is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Biotinylated Human DKK1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-5, hFc Tag with the EC50 of 66.8ng/ml determined by ELISA (QC Test).
Amino Acid Range: Thr32-His266

Catalog #DKK-HM401B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human DKK1 Protein (DKK-HM401B) Summary

Product Description	Recombinant Biotinylated Human DKK1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Thr32-His266.
Product Background	Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Dkk antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human DKK1 Protein (DKK-HM401B) Specifications/Details

Protein	DKK1
Synonyms	Dickkopf-1; Dkk1; SK
Accession	O94907
Species	Human
Biotinylated	yes
Amino Acid Range	Thr32-His266
Molecular Weight	The protein has a predicted MW of 28.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human DKK1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-5, hFc Tag with the EC50 of 66.8ng/ml determined by ELISA (QC Test).
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human DKK1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-5, hFc Tag with the EC50 of 66.8ng/ml determined by ELISA (QC Test).
	Immobilized Biotinylated Human DKK1, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 0.34ug/ml determined by ELISA.
	Immobilized Anti-DKK1 Antibody, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human DKK1, His Tag with the EC50 of 3.1ng/ml determined by ELISA.
	Biotinylated Human DKK1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human DKK1 is greater than 90% as determined by SEC-HPLC.