Product Description |
Recombinant Biotinylated Human DKK1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Thr32-His266. |
Product Background |
Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Dkk antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. |
Product Category |
Recombinant Protein |
Target |
DKK1 |
Synonyms |
Dickkopf-1; Dkk1; SK |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Thr32-His266 |
Molecular Weight |
The protein has a predicted MW of 28.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Anti-DKK1 Antibody, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human DKK1, His Tag with the EC50 of 3.1ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Anti-DKK1 Antibody, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human DKK1, His Tag with the EC50 of 3.1ng/ml determined by ELISA. |
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Immobilized Biotinylated Human DKK1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-5, hFc Tag with the EC50 of 66.8ng/ml determined by ELISA (QC Test). |
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Immobilized Biotinylated Human DKK1, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 0.34ug/ml determined by ELISA. |
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Biotinylated Human DKK1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human DKK1 is greater than 95% as determined by SEC-HPLC. |
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