Biotinylated Human DLL3 Domain (311-492) Protein, Ultra Low Endotoxin (DLL-HM4D3B-UL)

SKU: DLL-HM4D3B-UL-100UG

Biotinylated Human DLL3 Domain (311-492) Protein, Ultra Low Endotoxin (DLL-HM4D3B-UL)

SKU: DLL-HM4D3B-UL-100UG
Biotinylated Human DLL3 Domain (311-492) Protein, Ultra Low Endotoxin (DLL-HM4D3B-UL)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Biotinylated Human DLL3 (311-492), His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA.
  • Amino Acid Range: Val311-Leu492
Catalog #DLL-HM4D3B-UL
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Size
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Biotinylated Human DLL3 Domain (311-492) Protein, Ultra Low Endotoxin (DLL-HM4D3B-UL) Summary

Product Description

 Recombinant Biotinylated Human DLL3 Domain (311-492) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Val311-Leu492.

Product Background

 Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. DLL3 inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity).

Product Category

 Recombinant Protein / Antibody discovery / ADC targets

Biotinylated Human DLL3 Domain (311-492) Protein, Ultra Low Endotoxin (DLL-HM4D3B-UL) Specifications/Details

Protein
 DLL3
Synonyms
 Delta3; DLL3; Pudgy; SCDO1; SCDO1delta3
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Val311-Leu492
Molecular Weight
 The protein has a predicted MW of 22.69 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human DLL3 (311-492), His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA.
Product Tag
 C-His-Avi

Fluorophore

 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human DLL3 (311-492), His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA. Immobilized Biotinylated Human DLL3 (311-492), His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA.
Biotinylated Human DLL3 Domain (311-492) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human DLL3 Domain (311-492) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...
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