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Biotinylated Human DLL3 Domain (311-492) Protein, Ultra Low Endotoxin (DLL-HM4D3B-UL) Summary
Product Description |
Recombinant Biotinylated Human DLL3 Domain (311-492) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Val311-Leu492. |
Product Background |
Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. DLL3 inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity). |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Biotinylated Human DLL3 Domain (311-492) Protein, Ultra Low Endotoxin (DLL-HM4D3B-UL) Specifications/Details
Protein |
DLL3 |
Synonyms |
Delta3; DLL3; Pudgy; SCDO1; SCDO1delta3 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Val311-Leu492 |
Molecular Weight |
The protein has a predicted MW of 22.69 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human DLL3 (311-492), His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human DLL3 (311-492), His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA. |
|
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Biotinylated Human DLL3 Domain (311-492) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
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