Name: Biotinylated Human DNAM-1/CD226 Protein (DAM-HM101B)
Brand: KactusBio
SKU: DAM-HM101B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human DNAM-1/CD226 Protein (DAM-HM101B)

Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human DNAM-1, His-Avi Tag with an affinity constant of 264nM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Human DNAM-1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Human CD155, mFc Tag with the EC50 of 11.0ng/ml determined by ELISA (QC Test).

Biotinylated Human DNAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human DNAM-1 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by SEC-HPLC
Activity: Immobilized Biotinylated Human DNAM-1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Human CD155, mFc Tag with the EC50 of 11.0ng/ml determined by ELISA (QC Test). The affinity constant of 264nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Glu19-Asn247

Catalog #DAM-HM101B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human DNAM-1/CD226 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu19--Asn247.
Product Background	DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	DNAM-1/CD226
Synonyms	CD226 molecule; CD226; DNAM1; PTA1; TLiSA1
Accession	Q15762
Species	Human
Biotinylated	yes
Amino Acid Range	Glu19-Asn247
Molecular Weight	The protein has a predicted MW of 28.9 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human DNAM-1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Human CD155, mFc Tag with the EC50 of 11.0ng/ml determined by ELISA (QC Test). The affinity constant of 264nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by SEC-HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human DNAM-1, His-Avi Tag with an affinity constant of 264nM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human DNAM-1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Human CD155, mFc Tag with the EC50 of 11.0ng/ml determined by ELISA (QC Test).
	Biotinylated Human DNAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human DNAM-1 is greater than 95% as determined by SEC-HPLC.