Biotinylated Human DNAM-1/CD226 Protein (DAM-HM101B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human DNAM-1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Human CD155, mFc Tag with the EC50 of 11.0ng/ml determined by ELISA (QC Test). The affinity constant of 264nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Glu19-Asn247

Catalog: DAM-HM101B

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Product Description Recombinant Biotinylated Human DNAM-1/CD226 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu19--Asn247.

Product Background

 DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.

Product Category

 Recombinant Protein

Target

 DNAM-1/CD226
Synonyms CD226 molecule; CD226; DNAM1; PTA1; TLiSA1

Gene/Protein ID

 Q15762

Species

 Human

Exact Sequence

 Glu19-Asn247
Molecular Weight The protein has a predicted MW of 28.9 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Biotinylated Human DNAM-1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Human CD155, mFc Tag with the EC50 of 11.0ng/ml determined by ELISA (QC Test). The affinity constant of 264nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

 C-His-Avi

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human DNAM-1, His-Avi Tag with an affinity constant of 264nM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human DNAM-1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Human CD155, mFc Tag with the EC50 of 11.0ng/ml determined by ELISA (QC Test).
The purity of Biotinylated Human DNAM-1 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human DNAM-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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