Biotinylated Human DNAM-1/CD226 Protein (DAM-HM101B)
Catalog: DAM-HM101B
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Product Description | Recombinant Biotinylated Human DNAM-1/CD226 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu19--Asn247. |
Product Background |
DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2. |
Product Category |
Recombinant Protein |
Target |
DNAM-1/CD226 |
Synonyms | CD226 molecule; CD226; DNAM1; PTA1; TLiSA1 |
Accession | |
Species |
Human |
Amino Acid Range |
Glu19-Asn247 |
Molecular Weight | The protein has a predicted MW of 28.9 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Biotinylated Human DNAM-1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Human CD155, mFc Tag with the EC50 of 11.0ng/ml determined by ELISA (QC Test). The affinity constant of 264nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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