Name: Biotinylated Human DPPIV/CD26 Protein (DPV-HM426B)
Brand: KactusBio
SKU: DPV-HM426B-100UG
Price: 578.0 USD
Availability: InStock

Biotinylated Human DPPIV/CD26 Protein (DPV-HM426B)

Immobilized Biotinylated Human DPPIV, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-DPPIV Antibody, hFc Tag with the EC50 of 7.6ng/ml determined by ELISA.

Biotinylated Human DPPIV on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.

The purity of Biotinylated Human DPPIV is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 90% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human DPPIV, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-DPPIV Antibody, hFc Tag with the EC50 of 7.6ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is >8500 pmol/min/µg.
Amino Acid Range: Asn29-Pro766

Catalog #DPV-HM426B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human DPPIV/CD26 Protein (DPV-HM426B) Summary

Product Description	Recombinant Biotinylated Human DPPIV/CD26 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains Asn29-Pro766.
Product Background	CD26/dipeptidyl peptidase (DPP)IV is a membrane-bound protein found in many cell types of the body, and a soluble form is present in body fluids. There is longstanding evidence that various primary tumors and also metastases express DPPIV/CD26 to a variable extent.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human DPPIV/CD26 Protein (DPV-HM426B) Specifications/Details

Protein	DPPIV/CD26
Synonyms	ADCP2; CD26; DPPIV; DPP4; ADABP; TP103; EC 3.4.14.5
Accession	P27487
Species	Human
Biotinylated	yes
Amino Acid Range	Asn29-Pro766
Molecular Weight	The protein has a predicted MW of 88.27 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human DPPIV, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-DPPIV Antibody, hFc Tag with the EC50 of 7.6ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is >8500 pmol/min/µg.
Product Tag	N-His-Avi
Expression System	HEK293
Purity	> 90% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human DPPIV, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-DPPIV Antibody, hFc Tag with the EC50 of 7.6ng/ml determined by ELISA.
	Biotinylated Human DPPIV on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
	The purity of Biotinylated Human DPPIV is greater than 95% as determined by SEC-HPLC.